SNP - This stands for 'single nucleotide polymorphisms '
It is a single nucleotide variation in the DNA of the genome of the individual. Each of these variations are more than 1% in the population.
RFLP - Restrictions fragment length polymorphisms- it is a technique, particularly used in molecular biology, to study the variations in the homologous DNA sequences.
This method hepls to find minor differences between individuals ,populations and species.
These sequences also help to find particular gene sequences in the genome.
HOW CAN THESE BE USED TO FIND THE PARTICULAR GENE OF INTEREST.
These act by trail and error method. And this also requires particular probes and enzymes.
describe a SNP and a RFLP. How can these be used to locate a gene of...
How can Southern Blotting, a type of RFLP, be used when dealing with plasmid DNA? Do you use the plasmid as a molecular probe?
Anatomically describe how to locate the rhomboid muscles on the cat. Anatomically describe how to locate the brachioradialis muscle on the cat. Anatomically describe how to locate the four hamstring muscles on the cat. Anatomically describe how to locate and determine the four abdominal muscles on the cat.
Questions? BIOTECHNOLOGY: Describe and explain what restriction enzymes are? what their important characteristic are, and how they work, and how they can be used for forensic applications( RFLP analysis) Explain and understand gel electrophoresis, how does IT sort Dna by size? You should understand what PCR is and the basic of how IT works and its forensic applications (STR analysis) of present with a gel fragment pattern, you should be able to interpret it.
. If an adenoviral vector is used for gene therapy, why might it be necessary to give the patient multiple rounds? 4. How can a “suicide gene” be used to treat cancer? 5. Rexin-G is an effective vector for tumor treatment. Describe its 2 primary modes of action.
Describe how homologous recombination can change DNA sequences by gene conversion vs. how it can cause deletions/insertions.
8. Explain how SNP genotyping can improve patient outcomes when prescribing drugs.
Describe and outline the structural components of a gene, include all regulatory regions that can impact transcription initiation. Then add the chromatin structure, making sure to describe the structure and role of the nucleosome. Now, describe the process of how a gene is actively expressed starting with transcription initiation and ending with a protein product (be specific about each step). Lastly, describe three different mechanisms of regulation of this process and the possible outcomes of each.
Using SNP genotyping you have determined that a focal individual is a double heterozygote for two loci, whereby for locus 1 it has the genotype C1T1 and for locus 2 it has the genotype A2G2. Describe an experimental design that will allow you to determine whether that focus individualis C1_A2/T1_G2 or C1_G2/T1_A2. (The corresponding question on the mini test will supply an experiment that is used and give you results but if you understand how to set up the experiment...
What are the three basic functions of money? Describe how rapid inflation can undermine money’s ability to perform each of the three functions. Answer each part of the question, and give details. What "backs" the money supply in the United States? What determines the value (domestic purchasing power) of money? How does the purchasing power of money relate to the price level? Who is the U.S. is responsible for maintaining money 's purchasing power?
Describe in great detail the regulatory system that was used to express the eyeless gene in flies. Your answer must name the two components of this system, as well as a description of how they work together.