Question

1) Which of the following is FALSE about RNA polymerase?It does NOT require a primer to begin synthesis

It synthesizes RNA 5’ to 3’

It catalyzes the formation of phosphodiester bonds

It only transcribes one of the two DNA strands for any particular gene

It only transcribes exons

2)Which of the following is not synthesized from, or does not use DNA as a template?

Introns

Poly A tail

Ribosomal binding site

Primase

RNA polymerase

3) Which of the following is TRUE of translation?

Translation is terminated when a release factor binds to the P site of the ribosome

Ribosomes move along the mRNA in the 5’ to 3’ direction

Anticodons bind to codons covalently

The first tRNA charged with met binds to the A site of the ribosome

Amino acids are attached to the 5’ end of the tRNA

Answer 1

1. It only transcribes exons

This statement is incorrect because RNA polymerase transcribes all the nucleotides present on DNA molecule, irrespective of the nucleotides being exons or introns.

RNA polymerase is a DNA dependent RNA polymerizing enzyme. It uses DNA are the template to carry out the polymerization of RNA. It is an important process in transcription which is the formation of RNA from DNA.

2. Poly A tail

Ribosome binding site is present on messenger RNA and is coded from DNA as a template.

Primase and RNA polymerase are protein enzymes which are also coded from DNA. They are folded from DNA to messenger RNA and then to proteins.

Introns are non coding sequences of DNA which are present in messenger RNA along with exons.

Poly A tail is formed in messenger RNA after messenger RNA is coded from DNA. Therefore this is not formed from DNA as a template. The addition of tail and Cap to messenger RNA is independent.

3. Ribosomes move along the mRNA in the 5’ to 3’ direction

First statement is incorrect because released after bind to A site of ribosome.

Third statement is incorrect because anti codon and codon interact with each other by hydrogen bonding.

Last statement is incorrect because amino acids are attached to 3 prime end of transfer RNA.

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