A spectrophotometer can be used to determine the activity of a(n) _________ by following the change...
A spectrophotometer can be used to determine the activity of a(n) _________ by following the change of absorbance over time.
A. Organelle
B. Enzyme
C. Protein
D. Cell
Homework Answers
B. Enzyme (The spectrophotometer follows the course of an enzyme reaction by observing the changes in the intensity of the light absorbed and scattered by the reacting solution)
Add Answer to:
A spectrophotometer can be used to determine the activity of
a(n) _________ by following the change...
Explain the concept of an organelle marker protein or enzyme. How can a marker enzyme/protein aid...
Explain the concept of an organelle marker protein or enzyme. How can a marker enzyme/protein aid in isolating and enriching a preparation for an organelle? A student prepares mitochondria from a cauliflower which has been stored at 4°C for three weeks and finds the SDH specific activity of their PMS is about the same as their mitochondrial fraction. The same protocol used on fresh cauliflowers showed a 10-fold higher specific activity in the mitochondrial fraction compared to the PMS. Can...
A) If your spectrophotometer can measure a maximum absorbance of 1.5, what is the maximum concentration...
A) If your spectrophotometer can measure a maximum absorbance of 1.5, what is the maximum concentration of Compound N (ε=6220 M-1cm-1) that you can measure without diluting? B) If you add 3mL of water to 1mL of Compound, mix and get an absorbance of 0.2, what is the concentration of the original solution?
4 aswered A spectrophotometer measures the transmittance, the absorbance, or both, of a particular wavelength of...
4 aswered A spectrophotometer measures the transmittance, the absorbance, or both, of a particular wavelength of light after it has passed through a liquid sample. Before the transmittance or absorbance of the sample is measured, a cuvette filled only with solvent, called the blank, is placed in the spectrophotometer. t of 1.00 Flag Select the step that is not necessary to complete before a cuvette is placed into the spectrophotometer. Select one: a. Rinse the cuvette with the sample solution,...
SDS-PAGE is often used to determine the location and orientation of membrane associated proteins. Commonly, experimental...
SDS-PAGE is often used to determine the location and orientation of membrane associated proteins. Commonly, experimental preparations of membranes are treated in a variety of ways in order to extract the proteins, which are then subjected to SDS-PAGE. These treatments will affect membrane proteins in different ways, based on how they are arranged in the membrane. One of the most common pre-treatments is by the digestive enzyme trypsin, which degrades polypeptide chains. Trypsin is too large to cross a membrane,...
Which of the following amino acid side chains would be classified as a polar neutral amino...
Which of the following amino acid side chains would be classified as a polar neutral amino acid? Which of the following names identifies an enzyme? substrate glycolysis phosphoglucose dehydrogenase A protease cleaves what bond in its substrate? phosphodiester hydrogen double peptide Monitoring protease activity in milk can be accomplished by using a spectrophotometer measuring what variable? diameter of clearing absorbance of light time concentration of milk
1. Carefully explain how to complete the table below to determine the SDH specific activity in...
1. Carefully explain how to complete the table below to determine the SDH specific activity in the post nuclear supernatant using the data supplied. Assume the same volumes were used as in the practical and tubes 7 and 9 had a negligible change in DCPIP concentration over 30 minutes. tube A600nm 5 mins A600am 35 mins AA600nm 5-35 mins A[DCPIP) UM 5-35 mins 1 tube umoles DCPIP.min volume of extract assayed (ml) activity umoles.min l.ml1 specific activity umoles.min-1.mg 1 1...
When measuring the growth curve of E. coli by spectrophotometer, the death phase is not normally...
When measuring the growth curve of E. coli by spectrophotometer, the death phase is not normally seen as a change in absorbance because a. the cells are dividing most rapidly at that stage b. the cells are still “gearing up” to start dividing before log phase c. the spectrophotometer inhibits the death phase, so cells stay in stationary phase d. dead cells absorb and diffuse light to nearly the same degree as live cells do e. live cells are black,...
please answer the following Microbiology question. Which of the following statements is FALSE? A. The activity...
please answer the following Microbiology question.
Which of the following statements is FALSE? A. The activity of GTPases is regulated by the association of GTP or GDP with the protein B. Proteolytic cleavage of proteins can activate their activity ° C. Phosphorylation by kinases is an irreversible covalent modification that alters protein function D. Ubiquitinylation of a protein can result in protein degradation, immune system regulation or cell division regulation
To minimize the uncertainty in the measured concentration of an unknown using a visible spectrophotometer, the...
To minimize the uncertainty in the measured concentration of an unknown using a visible spectrophotometer, the analyst should a. Use a wavelength exhibiting high absorptivity. b. Select a wavelength where only the analyte responds. c. Adjust the absorbance of the unknown so that it is close to the mean absorbances of the standards used to develop the calibration curve. d. All of the above.
Activity-Based Costing and Product Cost Distortion Digital Storage Concept Inc. is considering a change to activity...
Activity-Based Costing and Product Cost Distortion Digital Storage Concept Inc. is considering a change to activity-based product costing. The company produce two products, cell phones and tablet PC, in a single production department. The production department estimated to require 4,000 direct labor hours. The total indirect laboris budgeted to be $533,600 Time records from Indirect labor employees revealed that they spent 60% of their time setting up production runs and 60% of their time supporting actual production The following information...
4 aswered A spectrophotometer measures the transmittance, the absorbance, or both, of a particular wavelength of light after it has passed through a liquid sample. Before the transmittance or absorbance of the sample is measured, a cuvette filled only with solvent, called the blank, is placed in the spectrophotometer. t of 1.00 Flag Select the step that is not necessary to complete before a cuvette is placed into the spectrophotometer. Select one: a. Rinse the cuvette with the sample solution,...
SDS-PAGE is often used to determine the location and orientation of membrane associated proteins. Commonly, experimental preparations of membranes are treated in a variety of ways in order to extract the proteins, which are then subjected to SDS-PAGE. These treatments will affect membrane proteins in different ways, based on how they are arranged in the membrane. One of the most common pre-treatments is by the digestive enzyme trypsin, which degrades polypeptide chains. Trypsin is too large to cross a membrane,...
Which of the following amino acid side chains would be classified as a polar neutral amino acid? Which of the following names identifies an enzyme? substrate glycolysis phosphoglucose dehydrogenase A protease cleaves what bond in its substrate? phosphodiester hydrogen double peptide Monitoring protease activity in milk can be accomplished by using a spectrophotometer measuring what variable? diameter of clearing absorbance of light time concentration of milk
1. Carefully explain how to complete the table below to determine the SDH specific activity in the post nuclear supernatant using the data supplied. Assume the same volumes were used as in the practical and tubes 7 and 9 had a negligible change in DCPIP concentration over 30 minutes. tube A600nm 5 mins A600am 35 mins AA600nm 5-35 mins A[DCPIP) UM 5-35 mins 1 tube umoles DCPIP.min volume of extract assayed (ml) activity umoles.min l.ml1 specific activity umoles.min-1.mg 1 1...
please answer the following Microbiology question.
Which of the following statements is FALSE? A. The activity of GTPases is regulated by the association of GTP or GDP with the protein B. Proteolytic cleavage of proteins can activate their activity ° C. Phosphorylation by kinases is an irreversible covalent modification that alters protein function D. Ubiquitinylation of a protein can result in protein degradation, immune system regulation or cell division regulation
Activity-Based Costing and Product Cost Distortion Digital Storage Concept Inc. is considering a change to activity-based product costing. The company produce two products, cell phones and tablet PC, in a single production department. The production department estimated to require 4,000 direct labor hours. The total indirect laboris budgeted to be $533,600 Time records from Indirect labor employees revealed that they spent 60% of their time setting up production runs and 60% of their time supporting actual production The following information...
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