Homework Answers
The answer is (d), make sure the cuvette has exactly the same amount of solution so the readings are consistent.
A spectrophotometer is dependent on concentration. The solution will have the same concentration whatever the amount may be. So it's not necessary that the cuvette has exactly the same amount of solution every time. All the other three points are necessary.
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4 aswered A spectrophotometer measures the transmittance, the absorbance, or both, of a particular wavelength of...
How can the colouration (or lack thereof) of the solutions be explained? How would you expect...
How can the colouration (or
lack thereof) of the solutions be explained?
How would you expect DELTAO to change as the number of L ligands
about the metal increases or decreases, and why?
UJUS ML PULUUPIUIUMLILI There are several types of spectrophotometers. Be sure to ask the instructor if you are unsure how to use the one you are assigned. You will be measuring absorbances at different wavelengths so it is essential to know how to quickly adjust the settings...
4. Having prepared all of your solutions, take all five of them to the spectrophotometer which...
4. Having prepared all of your solutions, take all five of them to the spectrophotometer which will has been set up for you. 5. Make sure the 6. Rinse and fill the plastic cuvette with the first (lowest has been zeroed using RO water in the cuvette ) standard solution and place the cell in the instrument with the clear windows in the light path. Read the absorbance at 450 nm and write it down in the following table. 7....
Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification...
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Part C. Quantitative Spectroscopy Wavelength(A) Absorbance Amax-10 Amax 10 Wavelength used for Analysis in Part C:...
Part C. Quantitative Spectroscopy Wavelength(A) Absorbance Amax-10 Amax 10 Wavelength used for Analysis in Part C: Solution Concentration Absorbance % Transmittance Standard 1 Standard 2 Standard 3 Standard 4 Unknown 0.20 M Show an example calculation obtaining the molarity of the standards. a. Copper Standard 2: Obtain additional 0.20 M CuSO4 solution from the tray. Pipet 10 mL of 0.20 M CuSO4 solution into a 50 mL Erlenmeyer flask. Pipet 10 mL of DI water into the same tube and...
the guiding questions. the max absorbance is 460. Guiding questions complex turque . Compare the molar...
the
guiding questions. the max absorbance is 460.
Guiding questions complex turque . Compare the molar absorptivity coefficients you obtained with SCN and Fe" as limiting reagents. Would you expect them to be the same? Why or why not? (Week 1) • The accepted molar absorptivity coefficient for FeSCN is 7.0x10' M'cm''. Find the bias and relative bias of your values and suggest possible sources of errors. (Week 1) • Which molar absorptivity coefficient will you use to calculate the...
11. Determine the color and the absorbance of the light and dark cuvettes at time. a....
11. Determine the color and the absorbance of the light and dark cuvettes at time. a. Be quick when performing these steps; b. Mix/invert/wipe and insert each cuvette into the Smer-20 to determine absorbance at 550 nm (Asso) [make sure you have pre-blanked spectrophotometer). c. Determine the color/pH by comparing the color of the Co, indicator in your cuvette to the provided Indicator Color Guide. d. Start keeping track of time. e. Immediately re-cover the dark cuvette and position the...
-Give a brief background on the role of pigments in photosythensis. -state your hypothesis. -finish with...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
all questions EXPERIMENT 8 Spectrophotometric Study of an Equilibrium Reaction Since known amounts of iron(III) and...
all questions
EXPERIMENT 8 Spectrophotometric Study of an Equilibrium Reaction Since known amounts of iron(III) and thiocyanate will be mixed, and the concentration of the iron-thiocyanate complex determined spectrophotometrically, it will be possible to cal- culate the equilibrium constant for each of the three possible equilibrium reactions above. The reaction which gives a constant value of K, for all the solutions prepared will be the correct one under the experimental conditions. AGRERERERLER . 1 Procedure 1. Clean and dry five...
How do I make a line of best fit (regression) with the following data? I am...
How do I make a line of best fit (regression) with the
following data? I am aware of how to make it in Microsoft Excel,
but I am having trouble figuring out how to find my x value for
making the graph. Y value is absorbance, what do I use for x value
to calculate the slope?
Tube 0.5 mM sulfide standard (ml) Sulfide amount (umol) Sulfide reagent (ml) 0.0 4.9 0.11 0.00 0.2 FeCl3 dH2O to Absorbance 10ml total...
Beer’s Law Objective : We will explore an application of absorption spectroscopy using calibration curves and...
Beer’s Law Objective : We will explore an application of absorption spectroscopy using calibration curves and Beer’s Law. Use the “LAB : HOW TO…” link from the class website if you need help with how to use balance, Bunsen burner… and such. Introduction: You may write this information in your lab notebook for your own reference. It can’t be cut and pasted. Different solutions have different spectral properties. In this portion of the experiment those properties will be utilized to...
How can the colouration (or
lack thereof) of the solutions be explained?
How would you expect DELTAO to change as the number of L ligands
about the metal increases or decreases, and why?
UJUS ML PULUUPIUIUMLILI There are several types of spectrophotometers. Be sure to ask the instructor if you are unsure how to use the one you are assigned. You will be measuring absorbances at different wavelengths so it is essential to know how to quickly adjust the settings...
4. Having prepared all of your solutions, take all five of them to the spectrophotometer which will has been set up for you. 5. Make sure the 6. Rinse and fill the plastic cuvette with the first (lowest has been zeroed using RO water in the cuvette ) standard solution and place the cell in the instrument with the clear windows in the light path. Read the absorbance at 450 nm and write it down in the following table. 7....
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Part C. Quantitative Spectroscopy Wavelength(A) Absorbance Amax-10 Amax 10 Wavelength used for Analysis in Part C: Solution Concentration Absorbance % Transmittance Standard 1 Standard 2 Standard 3 Standard 4 Unknown 0.20 M Show an example calculation obtaining the molarity of the standards. a. Copper Standard 2: Obtain additional 0.20 M CuSO4 solution from the tray. Pipet 10 mL of 0.20 M CuSO4 solution into a 50 mL Erlenmeyer flask. Pipet 10 mL of DI water into the same tube and...
the
guiding questions. the max absorbance is 460.
Guiding questions complex turque . Compare the molar absorptivity coefficients you obtained with SCN and Fe" as limiting reagents. Would you expect them to be the same? Why or why not? (Week 1) • The accepted molar absorptivity coefficient for FeSCN is 7.0x10' M'cm''. Find the bias and relative bias of your values and suggest possible sources of errors. (Week 1) • Which molar absorptivity coefficient will you use to calculate the...
11. Determine the color and the absorbance of the light and dark cuvettes at time. a. Be quick when performing these steps; b. Mix/invert/wipe and insert each cuvette into the Smer-20 to determine absorbance at 550 nm (Asso) [make sure you have pre-blanked spectrophotometer). c. Determine the color/pH by comparing the color of the Co, indicator in your cuvette to the provided Indicator Color Guide. d. Start keeping track of time. e. Immediately re-cover the dark cuvette and position the...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
all questions
EXPERIMENT 8 Spectrophotometric Study of an Equilibrium Reaction Since known amounts of iron(III) and thiocyanate will be mixed, and the concentration of the iron-thiocyanate complex determined spectrophotometrically, it will be possible to cal- culate the equilibrium constant for each of the three possible equilibrium reactions above. The reaction which gives a constant value of K, for all the solutions prepared will be the correct one under the experimental conditions. AGRERERERLER . 1 Procedure 1. Clean and dry five...
How do I make a line of best fit (regression) with the
following data? I am aware of how to make it in Microsoft Excel,
but I am having trouble figuring out how to find my x value for
making the graph. Y value is absorbance, what do I use for x value
to calculate the slope?
Tube 0.5 mM sulfide standard (ml) Sulfide amount (umol) Sulfide reagent (ml) 0.0 4.9 0.11 0.00 0.2 FeCl3 dH2O to Absorbance 10ml total...
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