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What was the purpose of the second quadrant of the agglutination assay containing only the reagent?...

What was the purpose of the second quadrant of the agglutination assay containing only the reagent? In an agglutination testing

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The purpose of the second quadrant of the agglutination assay containing the reagent only is to check for any auto-agglutination that might be present in the reagent which can lead to incorrect results (false-positives).

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