(1 bookmark) How can I calculate the Concentraition (M) with this table? (Beer- Lambert Law) concentration(M)...
(1 bookmark)
How can I calculate the Concentraition (M) with this table? (Beer- Lambert Law)
| concentration(M) | Absorbance(no units) | Lamda(max)(nm) | |
| reference | 0 | 0 | |
| #1 | 1.01 | 640nm | |
| #2 | 0.50 | 640nm | |
| #3 | 0.26 | 640nm | |
| #4 | 0.12 | 640nm | |
| #5 | 0.08 | 640nm | |
| Unknown | 0.72 | 640nm |
The cuvette is 1cm.
and the first experiment(#1, I use the 20mL of 1*10^-5 M blue
dye.
Each time, I use the pipet to transfer 10mL of solution from #1 and
add 10mL water.
And don't know the first condition about the unknown.
I don't know how to use the lambda max to the Beer-Lambert
Law.
Help me please .
Homework Answers
Add Answer to:
(1 bookmark)
How can I calculate the Concentraition (M) with this table?
(Beer- Lambert Law)
concentration(M)...
the Lambert-Beer law relates the absorption of light with the concentration and physical properties of a...
the Lambert-Beer law relates the absorption of light with the concentration and physical properties of a substance. 5 µl of a stock protein solution was diluted to 700 µL with water in a cuvette. the absorbance at 280 nm was 0.24. the extinction coefficient ξ for the protein is 10.3 mM^-1cm^-1. what is the molar concentration of the original stock protein solution? what is the concentration of the protein solution in µg/µL if the protein molar mass is 35348 Da.
is there anyway to calculate the absorbance from beer-lambert law? Extinction coefficient = 82 mm-1 concentration...
is there anyway to calculate the absorbance from beer-lambert law? Extinction coefficient = 82 mm-1 concentration = 3.2 mw = 17 dA
Beer lambert law. Simple Q Can some one find the molar absorbitiy "e" for the 1,1'-diethyl 2,2'-cyanine...
Beer lambert law. Simple Q
Can some one find the molar absorbitiy "e" for
the 1,1'-diethyl 2,2'-cyanine iodide. ( SEE DATA BELLOW
AND CALCULATIONS) and the theoretical value and explain where there
is a diffrence between them. Thank you so much.
3) Compare the experimental and literature values of ε (dye I only). Do you notice any discrepancies between these values? Dilution 1 Dilution2 Absorbance Dilution 3 Dilution 4 Dilutions 350 400 450 500 550 600 650 700 750 -0.2 Wavelength...
solve all 3 dont waste my answer 1. Check Beer's Law by plotting absorbance vs molar concentration using 2....
solve all 3 dont waste my answer
1. Check Beer's Law by plotting absorbance vs molar concentration using 2. Is Beer's Law valid for this system? Explain your answer. 3. If Beer's Law is valid, the value of "E" should be constant. Use A-ECPL or E = A/(EL) and the data from Table II to calculate values of E for the five solutions below. The value of L is normally 1 cm so E - A/C in this experiment. Table...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...
Prelaboratory assignment Exp. 4 Beer’s Law 1.Calculate the Concentration of the Crystal Violet Solutions in Table...
Prelaboratory assignment Exp. 4 Beer’s Law
1.Calculate the Concentration of the Crystal Violet Solutions in
Table 1 of the Procedureusing Vinitial x Minitial = Vdiluted x
Mdiluted (Note: Vdiluted = 15 mL). Record the concentrationsin
Table 1 AND in the Data table in the report sheet.
2.Plot the following data as absorbance vs concentration of X in
excel
Absorbance, A Molar Concentration of X
0.045 3.0 x 10-4 mol/L
0.097 6.2 x 10-4 mol/L
0.14 9.0 x 10-4 mol/L...
I need help on how to find the atmospheric pressure from the scatter plot and trend...
I need help on how to find the atmospheric pressure from the
scatter plot and trend line I made with the results from my Boyle's
Law lab. I don't know if my scatter plot is correct. I need the
atmospheric pressure in order to fill in Table 5 and answer some
questions.
24. Use the data in Data Table 1 and Data Table 3 to create a
scatter-plot graph of 1/Average Volume vs. Pressure; with
“1/Average Volume (mL-1)” on the...
Beer lambert law. Simple Q
Can some one find the molar absorbitiy "e" for
the 1,1'-diethyl 2,2'-cyanine iodide. ( SEE DATA BELLOW
AND CALCULATIONS) and the theoretical value and explain where there
is a diffrence between them. Thank you so much.
3) Compare the experimental and literature values of ε (dye I only). Do you notice any discrepancies between these values? Dilution 1 Dilution2 Absorbance Dilution 3 Dilution 4 Dilutions 350 400 450 500 550 600 650 700 750 -0.2 Wavelength...
solve all 3 dont waste my answer
1. Check Beer's Law by plotting absorbance vs molar concentration using 2. Is Beer's Law valid for this system? Explain your answer. 3. If Beer's Law is valid, the value of "E" should be constant. Use A-ECPL or E = A/(EL) and the data from Table II to calculate values of E for the five solutions below. The value of L is normally 1 cm so E - A/C in this experiment. Table...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...
Prelaboratory assignment Exp. 4 Beer’s Law
1.Calculate the Concentration of the Crystal Violet Solutions in
Table 1 of the Procedureusing Vinitial x Minitial = Vdiluted x
Mdiluted (Note: Vdiluted = 15 mL). Record the concentrationsin
Table 1 AND in the Data table in the report sheet.
2.Plot the following data as absorbance vs concentration of X in
excel
Absorbance, A Molar Concentration of X
0.045 3.0 x 10-4 mol/L
0.097 6.2 x 10-4 mol/L
0.14 9.0 x 10-4 mol/L...
I need help on how to find the atmospheric pressure from the
scatter plot and trend line I made with the results from my Boyle's
Law lab. I don't know if my scatter plot is correct. I need the
atmospheric pressure in order to fill in Table 5 and answer some
questions.
24. Use the data in Data Table 1 and Data Table 3 to create a
scatter-plot graph of 1/Average Volume vs. Pressure; with
“1/Average Volume (mL-1)” on the...
Most questions answered within 3 hours.
-
Water can decompose into H2 and O2 via electrolysis. How many
moles of H2 could theoretically...
asked 2 minutes ago -
How many moles of oxygen would be consumed during the aerobic
oxidation of one mole of...
asked 19 minutes ago -
Choose an enzyme and tell its function and what substrate it
works on.
asked 17 minutes ago -
You are helping the CFO of a manufacturing company assess
whether the firm should embark on...
asked 13 minutes ago -
a) Given these data for the reaction , write the rate-law
expression.
(Use k for the...
asked 18 minutes ago -
Compare two countries how the culture
of those country enhances or impedes your understanding of the...
asked 19 minutes ago -
Identify the incorrect statement.
A. An increase in mol does not increase the average force of...
asked 21 minutes ago -
In broadcast authentication, a senderaims to send
oneauthentication code for multiple receivers to verify. Explain
why...
asked 20 minutes ago -
A student studying for a vocabulary test knows the meanings of
16 words from a list...
asked 52 minutes ago -
List the most common organic functional groups that function as
acids or bases. Write an equation...
asked 33 minutes ago -
1) A protein is 435 amino acids long, which of the genes below
COULD NOT code...
asked 36 minutes ago -
What do the phenomena of overshadowing, the CS preexposure
effect, and relative validity of cues have...
asked 1 hour ago