What would you see when you look at the results of the genomic and cDNA PCRs...
What would you see when you look at the results of the genomic and cDNA PCRs if the mRNA from the CL2 gene underwent alternative splicing?
Homework Answers
Generally, when we speak about the genomic of DNA, it consists of the CL2 gene and it is a single entity. Now, upon alternate splicing there are several mRNAs which are produced from the same genomic DNA. During the process of alternative splicing, it is a process of regulated gene expression and hence a single gene encodes for many proteins. Hence, there are particular exons for gene which are excluded within the mRNA gene frame and some are not. Upon doing a genomic and cDNA PCRs, if the mRNA underwent alternative splicing for the CL2 gene, it would produces gene fragments of different fragment length in the PCR.
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