To be able to clone a intron free gene from E. coli using PCR, you would...
To be able to clone a intron free gene from E. coli using PCR, you would need to use ____ as template.
|
A. cDNA generated from E. coli |
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|
B. chromosomal DNA |
||
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C. Chromosomal DNA or cDNA, because the bacteria does not have introns |
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|
D. mature mRNA |
Homework Answers
Answer:
E.coli is a bacteria and it it does not have any introns in its genome.
So, all the genes present in E.coli are intron free and hence does not require mRNA or cDNA.
Chromosomal DNA is sufficient to amplify any gene using PCR and hence the answer is
B. Chromosomal DNA
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