Question

not sure what the correct answers are

To be export-ready, an mRNA molecule must: have its 5' cap removed be bound by EJCS have its poly-A tail removed have at least half of its introns removed od 80e covou The extension temperature used during PCR is determined by: the length of the PCR product you want to generate the DNA polymerase that is being used the annealing temperature of your primers x the source of your template DNA (eg, genomic DNA VS. DNA) * The proteosome... is a cytosolic structure involved in protein synthesis is a cytosolic structure involved in protein folding is a cytosolic structure involved in degradation of misfolded proteins is a cytosolic structure involved in the synthesis of ubiquitin none of the above A OŠ RT-PCR allows us to... make mRNA from a DNA template amplify the coding region of a gene from a mature mRNA amplify the just the coding region of a gene from chromosomal DNA DNA use reverse transcription to generate billions of copies of DNA The major reason you are able to predict the size of the PCR product generated in your reaction is... you can't predict this all PCR products are roughly the same size you know the sequence of the primers utilized because Taq DNA polymerase is optimized for a specific size PCR product After translation, a protein will either: form protein aggregates correctly fold with help of a molecular chaperone incompletely fold and be degraded by the proteasome correctly fold without help any of the above are possible MO Which of the following is not a type of DNA binding domain? SH2 zinc finger leucine zipper helix-loop-helix

Answer 1

19. Option b

Export ready mRNA signifies that the mRNA is associated with the appropriate set of proteins like cap-binding complex (CBC), the exon junction complex (EJC), and poly-A-binding protein. Spliceosome and its component proteins must be absent.

20. Option b

The temperature of extension usually depends on the optimum temperature of the polymerase used in the PCR. At an increased temperature (72 °C), the activity of the polymerase is optimum for primer extension.

21. Option c

Proteasomes cytosolic protein complexes that are reported to degrade the misfolded or damaged proteins by breaking the peptide bonds (proteolysis).

22. Option d

RT-PCR technique uses the principle of reverse transcription of RNA into DNA or cDNA. In this way, it amplifies specific DNA targets by PCR.

23. Option c

PCR product size can be predicted from the knowledge of the sequence of primers used for the amplification process. The primers used are highly specific for the gene of interest.

24. Option e

The protein formed after translation can fold on their own due to their intrinsic properties, or can take the help of the chaperons. The proteins can also misfold or form aggregates. The misfolded proteins are later degraded by the proteosome or are accumulated and can cause different disease conditions in some cases.

25. Option a

The SH2 (Src Homology 2) domain is a protein domain that is structurally conserved. It helps in docking/attachment of phosphorylated tyrosine residues on other proteins.