Question

4. You have partial transcriptomis data for a human gene and want to revise the annotation in the human genome. TITLE_EL Your data is shown below. Exons are boxes and introns are dotted lines. a. Which introns are constitutive? b. Which transcripts show an intron retention? c. Which transcripts show competing 5' splice sites? Which transcripts show competing 3' spice sites? e. If mRNA isoform 1 is used to annotate the genome of this gene, does it best represent all mature mRNA isoforms presented here? Is there anything you would recommend changing in the annotation of this gene based on your data?

Answer 1

A. That lie between two consecutive exons, are consecutive introns. 1-4 (vertically), and 2 ( horizontally,)

a Inteons aepe constitutive : - which are in Between two consecative rexons, 1-4 (vertically 2 horizontally (1-42. ure Pro anshura -20 Iostrons Retentino Alternative splicing a-f(vertically, 4 Chonzontally I b sob orra o Infyons Retention to . C. Donar site (s'end of introns con - Acceptor site (alend of inte DNA annotation throcess o the location and ot identit of genegand codine regina dregion of genome- it inrolves mapping such as protein coding gene JJ

B. Intron retention- Alternative splicing, 1-7 ( vertically), 4 ( horizontally).

C. Donar site (5' end)

D. Acceptor site ( 3' end).

E. Dna annotation process of identifying location of gene and coding region of genome .it involves mapping , such as protein coding gene.

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