You are working with a mammalian cell that has K+ leak channels, Na+ leak channels, and...
You are working with a mammalian cell that has K+ leak channels, Na+ leak channels, and Na+ /K+ ATPase. In the extracellular solution, you use NaCl and KCl in which both the Na+ and K+ ions were radioactively labeled. The ion concentrations are as follows: inside [K+ ] = 150 mM, inside [Na+ ] = 10 mM, outside [K+ ] = 15 mM, outside [Na+ ] = 140 mM. a.
When the Na+ ions are at electrochemical equilibrium, what is the electrical charge of the cell (positive, neutral, or negative)? Why?
Homework Answers
The Na+ ions are at electrochemical equilibrium i.e ionic concentration of Na+ inside and outside of the cell are equal hence it won't affect the overall charge of the cell. In that case, the overall electrical charge of the cell should be Positive because of higher concentration of [K+ ] = 150 mM inside the cell as compared to outside of the cell membrane [K+ ] = 15 mM.
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You are working with a mammalian cell that has K+ leak channels,
Na+ leak channels, and...
1. Animal cells have a Na,K pump that couples the energy of ATP hydrolysis to transport 3 Na ions out of the cell and 2 K ions into the cell. Inside astrocytes, the concentration of Na is 20 mM and th...
1. Animal cells have a Na,K pump that couples the energy of ATP hydrolysis to transport 3 Na ions out of the cell and 2 K ions into the cell. Inside astrocytes, the concentration of Na is 20 mM and the concentration of K is 130 mM. The extracellular concentrations of Na and K are 145 mM and 5 mM, respectively. Calculate the energy required for the transport of Na and K , with this stoichiometry; assume that the cell...
Changes in K+ ion concentrations are often dangerous. Simply doubling the concentration of K+ in the...
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You have isolated the Na/K-ATPase in a semi-purified membrane system. Given higher concentrations of Na' out...
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Given these parameters, what is the AG for K ion transport into the frog muscle cell in the presence of Ach? Dr. Knowsalot smiles and nods. "Good," she says. "Now let's see whether K+ ions pass through the open Ach receptors into the cell. As you might remember, these channels are permeable to Na+, K+, and Ca2+. For a typical frog muscle cell, the concentration of K+ is roughly 124 mM inside the cell and 2.30 mM outside the cell."...
please explain steps and show work no pictures were provided. table 3.13 is given above. 3.46...
please explain steps and show work
no pictures were provided. table 3.13 is given above.
3.46 The cell membrane is covered with protein complexes called Na-K+ pumps. Each pump moves 3 Nations from the intracellular space to the extracellular milieu for every 2 K ions it moves into the cell. During normal function, the pumps work constantly. Under normal conditions, there is a gradient of Nat and K ions between the cell and the extracellular space; the concentrations of these...
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You have isolated the Na/K-ATPase in a semi-purified membrane system. Given higher concentrations of Na' out and higher K inside, addition of ATP to the inside leads to increases of Nat olutside and Kt inside as expected. If instead, you add the ATP to the outside of the membrane, you don't see any change in the ion levels. Why? • ATP acts as an irreversible inhibitor of the Na/K-ATPase if it binds on the exterior side. The active site for...
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please explain steps and show work
no pictures were provided. table 3.13 is given above.
3.46 The cell membrane is covered with protein complexes called Na-K+ pumps. Each pump moves 3 Nations from the intracellular space to the extracellular milieu for every 2 K ions it moves into the cell. During normal function, the pumps work constantly. Under normal conditions, there is a gradient of Nat and K ions between the cell and the extracellular space; the concentrations of these...
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