Explain the process of prokaryotic translation initiation, elongation, and termination and Describe the structure and regulation...
Explain the process of prokaryotic translation initiation, elongation, and termination and
Describe the structure and regulation of the lac operon and trp operon
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Explain the process of prokaryotic translation initiation,
elongation, and termination and
Describe the structure and regulation...
1.Describe and compare eukaryotic and prokaryotic flagella. 2.Describe post-translational and cotranslational transport. 3.Describe prokaryotic mRNA translation...
1.Describe and compare eukaryotic and prokaryotic flagella. 2.Describe post-translational and cotranslational transport. 3.Describe prokaryotic mRNA translation in detail, including all steps from start to finish, including all factors. 4.Describe the molecular events involved in regulation of the lac operon in response to both glucose and lactose levels, and transcript (mRNA) abundance regulation in the prokaryotic trp operon including attenuation. 5.Completely describe transcript (mRNA) abundance regulation in the prokaryotic trp operon, and discuss whether or not the attenuation mechanism of transcriptional...
Describe the process of Prokaryotic RNA transcription in molecular detail You must be able to describe the process and order of events in initiation, elongation, and termination. You must describe t...
Describe the process of Prokaryotic RNA transcription in molecular detail You must be able to describe the process and order of events in initiation, elongation, and termination. You must describe the catalytic/biological purpose function of the key proteins/elements in elongation -35 and -10 (Pribnow box) Promoters Sigma factors (does not matter which one) RNA Polymerase (including structure and subunits and mechanism) Rho-independent and rho-dependent termination How sequence composition affects promoter function
1. Describe the differences between prokaryotic and eukaryotic translation initiation. How does the ribosome find the...
1. Describe the differences between prokaryotic and eukaryotic translation initiation. How does the ribosome find the correct start codon and what proteins are involved in the process? please include the shine-dalgarno sequence in the answer. 2. Consider the following partial sequence of messenger RNA. The sequence below contains the code for a short, complete protein. 5 ́-UCCCCAGUCAUGGAGUCGUUAAUUAAAUGACCGGUGCGGAUCGUA - 3 ́ Using the codon chart (from your textbook or in the lecture slides), give the amino acid sequence of the protein...
Describe the steps (Initiation, Elongation and Termination) involved in translation of mRNA to generate a protein,...
Describe the steps (Initiation, Elongation and Termination) involved in translation of mRNA to generate a protein, including the all the important molecules involved and how they interact. Diagrams MUST be included in your answer. (Draw on some paper, then photograph and insert the drawing below.) You may add to your answer using bullet points if you find it easier, but make sure they are in the correct order!
6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7)...
6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7) During translation elongation in procaryotes, the interplay between EF-Tu and EF-TS regulate tRNA charging to the A site of the ribosome. Please describe in detail the sequence of events that take place during tRNAEFTU GTR ternary complex formation. Explain how the EF-Tu GDP that is discharged from the ribosome is converted to EF- Tu GTR that is the competent complex for translation elongation? What is...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of lac operon in E.coli. b. Role of CAP and cAMP the activator binding site c. Role of Lac Repressor and Operator and Inducer d. Role of Looping e. Effect of mutations f. Recessive v dominant mutations g. Complementing v noncomplementing mutations. Describe the regulation of the trp operon in E. coli. Describe at least three kinds of molecular switches involved in gene regulation. What...
Determine whether the following events occur during initiation, elongation, or termination. Initiation Elongation Termination in prokaryotes,...
Determine whether the following events occur during initiation, elongation, or termination. Initiation Elongation Termination in prokaryotes, the Shine-Dalgarno sequence pairs with rRN translocation occurs kinetic proofreading occurs IF2 dissociates an aminoacyl-tRNA enters the A site ribosome recycling factor displaces deacetylated tRNA from P site in E. coli, an initiation factor blocks the A site
b. What is the polycistron in the Lac operon? In the Trp operon? d. Name an aspect of...
b. What is the polycistron in the Lac operon? In the Trp operon? d. Name an aspect of prokaryotic gene regulation that can only occur in a prokaryote and not a eukaryote e. Provided the wildtype lac operon, give three different examples of mutations, what they target, and how they disrupt WT regulation of the operon. f. Describe how the RNA leader of the Trp operon can repress/induce gene translation
Prokaryotic Translation Order each picture set, from the initiation of translation to the termination of translation...
Prokaryotic Translation Order each picture set, from the initiation of translation to the termination of translation T себя у ч н н н н нонно N-с-с-N-с-с-N-с-с -с-с н R. o R. o R. o \ R, O н нннннно - C-C-N - C-C-N-C-C-N-с-с R 0 R₂0 R₂0 Ro Peptide bond H H H 0 N-C-C-N-C-C-N-C-C Ý ß o R o Rö | H H H H H Hà H H ) N-C-C-N-C-C-N-C-C-N-C-C H R o Ro R₂0 RO Before it...
15. Translation (RNA protein) has three main stages: initiation, elongation, and termination. a. Initiation occurs when...
15. Translation (RNA protein) has three main stages: initiation, elongation, and termination. a. Initiation occurs when the small ___________________ subunit binds to the ____ end of mRNA and is then joined by the large _________________ subunit (which has three sites called the A, P, and E sites). Once the complex is formed, the _______________ begins to read the mRNA in a ____ to ____ direction. When it reaches the first start codon (_________) a tRNA carrying the amino acid ______________________...
6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7) During translation elongation in procaryotes, the interplay between EF-Tu and EF-TS regulate tRNA charging to the A site of the ribosome. Please describe in detail the sequence of events that take place during tRNAEFTU GTR ternary complex formation. Explain how the EF-Tu GDP that is discharged from the ribosome is converted to EF- Tu GTR that is the competent complex for translation elongation? What is...
Determine whether the following events occur during initiation, elongation, or termination. Initiation Elongation Termination in prokaryotes, the Shine-Dalgarno sequence pairs with rRN translocation occurs kinetic proofreading occurs IF2 dissociates an aminoacyl-tRNA enters the A site ribosome recycling factor displaces deacetylated tRNA from P site in E. coli, an initiation factor blocks the A site
Prokaryotic Translation Order each picture set, from the initiation of translation to the termination of translation T себя у ч н н н н нонно N-с-с-N-с-с-N-с-с -с-с н R. o R. o R. o \ R, O н нннннно - C-C-N - C-C-N-C-C-N-с-с R 0 R₂0 R₂0 Ro Peptide bond H H H 0 N-C-C-N-C-C-N-C-C Ý ß o R o Rö | H H H H H Hà H H ) N-C-C-N-C-C-N-C-C-N-C-C H R o Ro R₂0 RO Before it...
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