1. ASP has an important role in hydrogen bonding with the His residue in the catalytic...
1. ASP has an important role in hydrogen bonding with the His residue in the catalytic triad of chymotrypsin. ASP and GLU have very similar properties as amino acids. Yet, mutation of ASP to GLU in the traid results in a 10^5 fold decrease in enzyme activity. Why do you think this could be the case?
Homework Answers
Catatylic triad in chymotrypsin include histidine, aspartic acid and serine. Each amino acid have specific role. The function of adaptic acid is to orient the histidine residue and make it better proton accept or that make it suitable for the catalytic mechanism of the enzyme. But if glutamic acid come in place of aspartic acid, GLU is not able to orient the histidine residue, because it have extra methyl group. This lead to less functioning of histidine and aspartic acid. Therefore, the enzyme activity get decreased.
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1. ASP has an important role in hydrogen bonding with the His
residue in the catalytic...
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The following questions are based on the papin mechanism below: Explain the role of each amino acid in the catalytic triad. Site directed mutagenesis replacing the active site asp in the papain protea...
The
following questions are based on the papin mechanism below:
Explain the role of each amino acid in the catalytic triad.
Site directed mutagenesis replacing the active site asp in the
papain protease with an ala showed that catalytic activity was not
significantly affected. Why would you think that asp is not so
critical in the cysteine proteases?
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2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...
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which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
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I need Summary of this Paper i dont need long summary i need What methodology they used , what is the purpose of this p...
I need Summary of this Paper i dont need long summary i need
What methodology they used , what is the purpose of this paper and
some conclusions and contributes of this paper. I need this for my
Finishing Project so i need this ASAP please ( IN 1-2-3 HOURS
PLEASE !!!)
Budgetary Policy and Economic Growth Errol D'Souza The share of capital expenditures in government expenditures has been slipping and the tax reforms have not yet improved the income...
An enzyme works well as a catalyst at a pH of 6.8. The pk of an essential catalytic residue is known to be 7.2 It is found that catalytic activity is significantly lower when pH= 8.1. What is likely the cause of the decrease in activity? Select all that apply. Select one or more: a. Glu or Asp must lose a proton to inactivate the protein, b. The protein has changed shape due to a change in charge. c. His...
The
following questions are based on the papin mechanism below:
Explain the role of each amino acid in the catalytic triad.
Site directed mutagenesis replacing the active site asp in the
papain protease with an ala showed that catalytic activity was not
significantly affected. Why would you think that asp is not so
critical in the cysteine proteases?
Hi R CH2 NH-R oe 0 hole oxyanion hole 0 oxyanion hole CH a)
Hi R CH2 NH-R oe 0 hole oxyanion...
A. Choose anly one correcet answer for each of the following questions (4 pts cach). AL Some and KM values are shown below for enzyme-substrate pairs. Which of the following enzymes is most efficient in converting the substrate into the product? b) kes.-4x10s s", KM-0.026 M d)k,,-5.7 x1o's", K-2x10s M c)人at-900 s", KM-2.5 × 10.5 M A2. Which of the following enzyme reaction mechanisms has multiple substrates? a) induced-fit e) Michaclis-Menton b) random sequential d) reversible covalent modification e) None...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
Cyw Ala Ατα Ile Ausn Lou Asp aly Lys Ser Which molecule below is asubunit of peptide chains a. Acetic acid b. Alanine O. ATP d. Gracil Report the charge on most aqueous aspartie acid molecules at pH-7. Report the approximate p values for the amino acid whose titration curve is shown below (note there may be more slots for answer than answers). A) Value 1 B) Value 2 c) Value 3 c) Value 4 The structure of a rare...
t How are donaturation and varovalo airterent a. Rydrolysis resulte to the formation of water. Hydrolysis only takes place in the cytopa c. Hydrolysis results in breakingole d. Hydrolysis results in breaking ovalent bo How is folding related to function? • Folding and function are not related.. Proper folding results in propertune 0. Proper folding results in reduced un roper folding results in arratiefunct Molecules that a t folding are called a. Foldons which cellular environment is . Cytoplasm c....
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
I need Summary of this Paper i dont need long summary i need
What methodology they used , what is the purpose of this paper and
some conclusions and contributes of this paper. I need this for my
Finishing Project so i need this ASAP please ( IN 1-2-3 HOURS
PLEASE !!!)
Budgetary Policy and Economic Growth Errol D'Souza The share of capital expenditures in government expenditures has been slipping and the tax reforms have not yet improved the income...
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