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1- what are the positive fermentation tests and negative fermentation tests of M. smegmatis bacteria? 2-...

1- what are the positive fermentation tests and negative fermentation tests of M. smegmatis bacteria?

2- what is the flow chart dichotomous key in the dermination of M. smegmatis?
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Answer #1

1, Mycobacterium smegmatis is a Gram-positive, acid-fast bacilli with an optimum growth temperature of 37oC. It is obligate aerobe and a harmless bacteria. It is a commonly used mycobacterial model system and a saprophyte.

A positive fermentation test is when a bacterium utilises the sugar present in the media and produce acid, gas as by-product which could be indicated by acid base indicators like phenol red whereas a negative result indicates that the bacteria is not capable of utilising the sugar and produce acid by-products.

The fermentation tests of Mycobacterium smegmatis,

Positive for

  1. Glucose
  2. arabinose
  3. ducitol
  4. fructose
  5. mannitol
  6. mannose
  7. sorbitol
  8. Inositol
  9. Xylose

ACid is usually the end product of the fermentation.

Negative

1, Lactose

2, Galactose

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