Question

1. A cancer study was conducted with female mice to determine the carcinogenic effects of food contaminated with 2-acetylaminofluorene. The animals were sacrificed and examined after two years. The raw data are shown below:

Dose in feed (mg/kg-day))	Number of animals	Number with lever neoplasm
0	762	20
30	2109	164
35	1361	128
45	888	98
60	758	118
75	587	118
100	297	76
150	314	126

Determine the CSF using linear model.

2. Using the slope factor determined in Problem 1, determine the concentration of 2-acetylaminofluorene that will give a risk of one tumor in 10⁶ exposed female mice.

3. Determine the risk of males working in a environment of formaldehyde at 3 ppm for 8 hr/day, 5 days/week, 48 weeks/year for 45 years, given the unit risk for inhalation is 0.027 /ppm. Calculate the cancer deaths in the following table:

Number of Workers	Cancer Deaths
100
1,000
10,000
100,000

Answer 1