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explain the purpose of the experiment where DNA from a maturing red blood cell and DNA...

explain the purpose of the experiment where DNA from a maturing red blood cell and DNA from an oviduct cell were both treated with DNAse I enzyme prior to using PCR to assay for the presence of globin and albumin genes.

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Answer #1

DNAse I is used for cleaving DNA's phosphodiester linkage.

Explanation

DNAse I ( Deoxyribonucleases I ) is an enzyme that is used to cleaves, degrades DNA after RNA isolation, cleanup the RNA prior to PCR, prevents clumping in handling cultured cells,creates DNA fragmented sequences for in-vitro recombination and identify protein binding sequence on DNA. DNAse I cleaves phosphodiester linkages adjacent to a pyrimidine nucleotide of DNA and yields 5'-phosphorylated di-, tri-, and oligonucleotide products.

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