You used E.coli, Staphylococcus aureus, and XL1-Blue II cells for the UV experiment. Which bacteria are...
You used E.coli, Staphylococcus aureus, and XL1-Blue II cells for the UV experiment. Which bacteria are the most UV sensitive and which bacteria are the least sensitive of these three? Give a detailed explanation!
Homework Answers
E.coli is most sensitive/less resistant because it has UV resistance of 20-40 seconds,
S. aureus is moderate sensitive/resistance because it has UV resistance of 10-20 minutes,
RecA cells is least sensitive/more resistant because it has UV resistance of 120 minutes.
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You used E.coli, Staphylococcus aureus, and XL1-Blue II cells
for the UV experiment. Which bacteria are...
would you expect XL1 Blue II cells to survive exposure to UV radiation? Why or why...
would you expect XL1 Blue II cells to survive exposure to UV radiation? Why or why not?
For a different experiment you are growing up Saccharomyces cerivisiae instead of E.coli. The measured OD...
For a different experiment you are growing up Saccharomyces cerivisiae instead of E.coli. The measured OD is 0.89. A) In order to calculate the concentration of the Saccharomyces cerivisiae culture, can you use the equation that you use to calculate the concentration of an E.coli culture? If yes or no, give a detailed explanation. B) Does E.coli or Saccharomyces cerivisiae have a shorter generation time? Give a detailed explanation!
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How many CFUs are expected if you plated 0.2 mL of a 104 dilution? (Hint: Consider the average number of cells per CFU) 18. At 65 minutes, the cells were transferred to a new medium in which the growth rate (H) was three times lower. What is the expected number of cells after 60 minutes of growth following the transfer? 19.By what factor did the...
Which antibiotic(s) is/are the most effective? Which antibiotic(s) is/are the least effective? The bacteria used was...
Which antibiotic(s) is/are the most effective? Which
antibiotic(s) is/are the least effective? The bacteria used was
E.coli and the antibiotics are shown in the diagram on the
right.
Results gentamycin penicillin Tetra polymixin Nalidixid cycline acid PAN SA Sulfa drug (sulfonamide)
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which...
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which antibiotics work against specific bacteria. a. Your patient comes in with a Staphylococcus aureus infection and you know that tetracycline kills S. aureus, but you want to prescribe the patient with the lowest concentration of tetracycline, which test would you conduct to find the lowest concentration that killed off S. aureus? b. Your patient comes in with a mystery bacterial infection. You want to...
and Lab Exer n this experiment you will evaluate effect of UV light on bacterial growth. UV light affects growth by interfering with DNA, resulting in thymine dimer formation. The dimerization...
and Lab Exer n this experiment you will evaluate effect of UV light on bacterial growth. UV light affects growth by interfering with DNA, resulting in thymine dimer formation. The dimerization is confined within adjacent thymine residues on same strand (intrastrand ). These dimmer lesions have consequence on DNA functions including interference in DNA replication, transcription etc, so UV exposure of cells can lead to lethal effects for bacterial multiplications. This forms the basis for UV method to control bacterial...
You are working on a DNA sequencing experiment on bacteria, which requires you to extrac DNA...
You are working on a DNA sequencing experiment on bacteria, which requires you to extrac DNA from bacterial cells. Why does it make sense to include a step in the protocol that adds RNAase, an enzyme that degrades RNA, to your DNA extraction mixture?
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
Antibiotic A: 0.5 kDa protein, targets peptidoglycan Antibiotic B: 20 kDa protein, targets peptidoglycan Antibiotic C:...
Antibiotic A: 0.5 kDa protein, targets peptidoglycan Antibiotic B: 20 kDa protein, targets peptidoglycan Antibiotic C: Cationic Antimicrobial Peptide (CAMP) Antibiotic D: Targets lipopolysaccharide With some more searching, you found the following results in your notebook. Percent Survival Percent Survival Antibiotic Antibiotic Control Control protoplast C. difficile protoplast Bacteria S. aureus E. coli V. cholera C. difficile S. aureus E. coli V. cholera Bacteria Percent Survival Percent Survival Antibiotic Antibiotic Control Control E.coli S. aureus Bacteria S. aureus E. colis...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells with an enzyme and ligated the fragments into plasmid vectors digested with the same enzyme. The plasmid you are using contains a gene for ampicillin resistance and an MCS located within the Lacz gene. For the ligation, you added three times as much of the digested plasmid as the insert. After introducing the ligated DNA into cells, you observe 200 blue colonies and 33...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How many CFUs are expected if you plated 0.2 mL of a 104 dilution? (Hint: Consider the average number of cells per CFU) 18. At 65 minutes, the cells were transferred to a new medium in which the growth rate (H) was three times lower. What is the expected number of cells after 60 minutes of growth following the transfer? 19.By what factor did the...
Which antibiotic(s) is/are the most effective? Which
antibiotic(s) is/are the least effective? The bacteria used was
E.coli and the antibiotics are shown in the diagram on the
right.
Results gentamycin penicillin Tetra polymixin Nalidixid cycline acid PAN SA Sulfa drug (sulfonamide)
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which antibiotics work against specific bacteria. a. Your patient comes in with a Staphylococcus aureus infection and you know that tetracycline kills S. aureus, but you want to prescribe the patient with the lowest concentration of tetracycline, which test would you conduct to find the lowest concentration that killed off S. aureus? b. Your patient comes in with a mystery bacterial infection. You want to...
and Lab Exer n this experiment you will evaluate effect of UV light on bacterial growth. UV light affects growth by interfering with DNA, resulting in thymine dimer formation. The dimerization is confined within adjacent thymine residues on same strand (intrastrand ). These dimmer lesions have consequence on DNA functions including interference in DNA replication, transcription etc, so UV exposure of cells can lead to lethal effects for bacterial multiplications. This forms the basis for UV method to control bacterial...
You are working on a DNA sequencing experiment on bacteria, which requires you to extrac DNA from bacterial cells. Why does it make sense to include a step in the protocol that adds RNAase, an enzyme that degrades RNA, to your DNA extraction mixture?
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
Antibiotic A: 0.5 kDa protein, targets peptidoglycan Antibiotic B: 20 kDa protein, targets peptidoglycan Antibiotic C: Cationic Antimicrobial Peptide (CAMP) Antibiotic D: Targets lipopolysaccharide With some more searching, you found the following results in your notebook. Percent Survival Percent Survival Antibiotic Antibiotic Control Control protoplast C. difficile protoplast Bacteria S. aureus E. coli V. cholera C. difficile S. aureus E. coli V. cholera Bacteria Percent Survival Percent Survival Antibiotic Antibiotic Control Control E.coli S. aureus Bacteria S. aureus E. colis...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells with an enzyme and ligated the fragments into plasmid vectors digested with the same enzyme. The plasmid you are using contains a gene for ampicillin resistance and an MCS located within the Lacz gene. For the ligation, you added three times as much of the digested plasmid as the insert. After introducing the ligated DNA into cells, you observe 200 blue colonies and 33...
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