For a different experiment you are growing up Saccharomyces cerivisiae instead of E.coli. The measured OD...
For a different experiment you are growing up Saccharomyces cerivisiae instead of E.coli. The measured OD is 0.89. A) In order to calculate the concentration of the Saccharomyces cerivisiae culture, can you use the equation that you use to calculate the concentration of an E.coli culture? If yes or no, give a detailed explanation. B) Does E.coli or Saccharomyces cerivisiae have a shorter generation time? Give a detailed explanation!
Homework Answers
A. The equation used to calculate the concentration of saccharomyces cervisea culture is same as bacteria.Normally Beers law is used to calculate concentration from absorbance.Beers law states that concentartion is directly prpotional to absorbance.
B. E.coli has a shorter generation time than yeast. E.coli generation time is 20 miutes and yeast generation time is 80 minutes. Time required for the organism to doubling in a cetain time period is called generation time.
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For a different experiment you are growing up Saccharomyces
cerivisiae instead of E.coli. The measured OD...
please use these results of a growth curve experiment to answer the following questions Questions 1)...
please use these results of a growth curve experiment to
answer the following questions
Questions 1) Plot the OD600 readings and cell concentration for each time point on a scatterplot with a line connecting the points. Have the measured data (OD600 and cell concentration) on the y-axis against the time on the x-axis using Excel (or other computer based data/graphing program). It is helpful to set the y-axis on a log scale for the cell concentration measurements. 2) Can you...
A friend of yours asks you to help him/her draw two bacterial growth curves. He/she gives...
A friend of yours asks you to help him/her draw two bacterial growth curves. He/she gives you the following information: the TA started both cultures (one was labeled culture �X� and the other culture �Y�) at 8:30am using the same overnight culture to inoculate fresh medium. After inoculation of the medium the OD was 0.14 for both cultures and both cultures were incubated at 37 oC. During class the following OD readings were obtained: for culture �X�: 0.19 at 9:50am,...
In this experiment, you will measure the rate of photosynthesis in isolated chloroplasts using the dye...
In this experiment, you will measure the rate of photosynthesis
in isolated chloroplasts using the dye 2,6-dichloroindophenol
(DPIP). The transfer of electrons during the light-dependent
reaction results in the production of H+. This H+ ion can reduce
DPIP, changing its color from blue to clear. The color can be
quantitatively measured with a spectrophotometer. However, in this
experiment, you will qualitatively analyze your data.
Write a detailed conclusion for your experiment. How did light
affect the time it took for...
In setting up your 3 kinetics experiment, you will be mixing different volumes the two reactants...
In setting up your 3 kinetics experiment, you will be mixing different volumes the two reactants (oxalic acid (H2C2O4) and potassium permanganate (KMnO4)). If you mix 10 ml of 0.02 M with 20 ml of 0.5 M oxalic acid, what is the concentration of H2C2O4 in the mixture? Hint: Use M1V1 = M2 V2.
In this experiment you will make a set of solutions containing different concentrations of CV+ by...
In this experiment you will make a set of solutions containing different concentrations of CV+ by mixing different volumes of a stock CV+ solution with water to give final volumes of 3.0 mL. The stock solution of CV+ has a concentration of 2.0x10^-5M and is dark purple. Use C1V1=C2V2 to calculate the volume of the stock 2.0x10^-5 M CV+ AND the volume of water to make a 3.0 mL sample of 4.0x10^-6 M CV+. Show calculations.
The equation is c= Q/mDeltat Please, can you give me you feedback on the following steps?...
The
equation is c= Q/mDeltat
Please, can you give me you feedback on the following steps? In order to investigate how salt concentration will affect the specific heat capacity elaborated the following experiment. I measured the amount of water and I record the initial temperature.Itake an amount x of salt and blend it in the water. I heat the water for 2 minutes and then I measure the final temperature. I do it for the water with salt and without...
2 Experiment: In the experiment conducted, the mass of the counter weight M was chosen to...
2 Experiment: In the experiment conducted, the mass of the counter weight M was chosen to be 50.0 +/- 0.1 g The mass of the rubber stopper was measured to be 12.0 +/- 0.1 g The stopper was spun at five different values of the radius (measured with a meter stick). For each value of r, the time to complete 10 full revolutions was measured with stopwatch. The following data was collected: 40.0 60.0 80.0 100.0 (cm) 1) 120.0 10.74...
Please, can you give me you feedback on the following steps? In order to investigate how...
Please, can you give me you feedback on the following steps? In order to investigate how salt concentration will affect the specific heat capacity I elaborated the following experiment. I measured the amount of water and I record the initial temperature. I take an amount x of salt and blend it in the water. I heat the water for 2 minutes and then I measure the final temperature. I do it for the water with salt and without salt. Then I...
Please use the same 15 questions to interview someone from a different backgrounds (culture/nationality/race/religion than your...
Please use the same 15 questions to interview someone from a different backgrounds (culture/nationality/race/religion than your own). Please try to use this assignment to learn as much as possible about our cultural differences (in Q/A format), and make sure to include information about your interviewee (his or her detailed background). 1-Describe your family (origin, class, education…………etc). 2-Your neighborhood-other factors: religion-disabilities-social class……. 3-The first time I became aware of my cultural background was when....do you identify yourself with any group? 4-As...
E this experiment follows a modified procedure designed to demonstrate only the lag and log phase...
e this experiment follows a modified procedure designed to demonstrate only the lag and log phases by using an indirect method foe measuring growth. The indirect method is based on atisie at intervals during the incubation period. Such a procedure does not lend itself to a regular laboratory curve will be plotted usin appears cloudier as the bacteria increase in number by cell division. A tube of bacte- r can measure the n liquid culture, the medium amount of light...
please use these results of a growth curve experiment to
answer the following questions
Questions 1) Plot the OD600 readings and cell concentration for each time point on a scatterplot with a line connecting the points. Have the measured data (OD600 and cell concentration) on the y-axis against the time on the x-axis using Excel (or other computer based data/graphing program). It is helpful to set the y-axis on a log scale for the cell concentration measurements. 2) Can you...
In this experiment, you will measure the rate of photosynthesis
in isolated chloroplasts using the dye 2,6-dichloroindophenol
(DPIP). The transfer of electrons during the light-dependent
reaction results in the production of H+. This H+ ion can reduce
DPIP, changing its color from blue to clear. The color can be
quantitatively measured with a spectrophotometer. However, in this
experiment, you will qualitatively analyze your data.
Write a detailed conclusion for your experiment. How did light
affect the time it took for...
The
equation is c= Q/mDeltat
Please, can you give me you feedback on the following steps? In order to investigate how salt concentration will affect the specific heat capacity elaborated the following experiment. I measured the amount of water and I record the initial temperature.Itake an amount x of salt and blend it in the water. I heat the water for 2 minutes and then I measure the final temperature. I do it for the water with salt and without...
2 Experiment: In the experiment conducted, the mass of the counter weight M was chosen to be 50.0 +/- 0.1 g The mass of the rubber stopper was measured to be 12.0 +/- 0.1 g The stopper was spun at five different values of the radius (measured with a meter stick). For each value of r, the time to complete 10 full revolutions was measured with stopwatch. The following data was collected: 40.0 60.0 80.0 100.0 (cm) 1) 120.0 10.74...
e this experiment follows a modified procedure designed to demonstrate only the lag and log phases by using an indirect method foe measuring growth. The indirect method is based on atisie at intervals during the incubation period. Such a procedure does not lend itself to a regular laboratory curve will be plotted usin appears cloudier as the bacteria increase in number by cell division. A tube of bacte- r can measure the n liquid culture, the medium amount of light...
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