Calculate the weight of the buffers you will use to make the buffers for Part A for all the buffer possibilities listed under procedures in Part A, step 1. In other words, how many grams do you need to make 100 mL of a 0.1 M buffer?
Part A:
Buffer | pKa1 | pKa2 | pKa3 | Formula Weight (g/mol) |
Acetate |
4.76 |
136.1 | ||
CAPS | 10.4 | 221.3 | ||
Citrate | 3.06 | 4.74 | 5.40 | 294.1 |
HEPES | 7.55 | 238.3 | ||
Phosphate | 2.12 | 7.21 | 12.32 | 142.0 |
Tricine | 8.15 | 179.2 | ||
TRIS | 8.3 | 121.1 |
Show work for at least one full buffer calculation!
Calculate the weight of the buffers you will use to make the buffers for Part A...
Not sure how to go about this problem, help please 2.) You need to make a buffer to use in the first step of the purification. You determine that a 0.1M buffer at a pH of 7.40 would be ideal. You choose one of the Good Buffers in the table shown below: Buffer MW pKa (20°C) Apka/ °C HEPES, free acid 238.3 7.55 -0.014 MOPS, free acid 209. 3 7.20 -0.006 Tris base 121.1 8.30 -0.031 MES, free acid 195.2...