Question

Calculate the weight of the buffers you will use to make the buffers for Part A...

Calculate the weight of the buffers you will use to make the buffers for Part A for all the buffer possibilities listed under procedures in Part A, step 1. In other words, how many grams do you need to make 100 mL of a 0.1 M buffer?

Part A:

Buffer pKa1 pKa2 pKa3 Formula Weight (g/mol)
Acetate

4.76

136.1
CAPS 10.4 221.3
Citrate 3.06 4.74 5.40 294.1
HEPES 7.55 238.3
Phosphate 2.12 7.21 12.32 142.0
Tricine 8.15 179.2
TRIS 8.3 121.1

Show work for at least one full buffer calculation!

0 0
Add a comment Improve this question Transcribed image text
Know the answer?
Add Answer to:
Calculate the weight of the buffers you will use to make the buffers for Part A...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • Not sure how to go about this problem, help please 2.) You need to make a...

    Not sure how to go about this problem, help please 2.) You need to make a buffer to use in the first step of the purification. You determine that a 0.1M buffer at a pH of 7.40 would be ideal. You choose one of the Good Buffers in the table shown below: Buffer MW pKa (20°C) Apka/ °C HEPES, free acid 238.3 7.55 -0.014 MOPS, free acid 209. 3 7.20 -0.006 Tris base 121.1 8.30 -0.031 MES, free acid 195.2...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT