You have 42 nmoles of a lyophilized (dried) oligonucleotide ( a short stretch of DNA). How...

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You have 42 nmoles of a lyophilized (dried) oligonucleotide ( a short stretch of DNA). How...

You have 42 nmoles of a lyophilized (dried) oligonucleotide ( a short stretch of DNA). How much volume in μL do you need to make a 100 μM solution?

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Answer 1

Answer #1

Ans :

Number of moles = 42 nmoles = 42 x 10^-9 mol

Molarity = 100 = 10^-4 M

M = number of mol of solute / volume of solution in L

putting values :

10^-4 M = 42 x 10^-9 mol / V

V = 42 x 10^-5 L

So the volume in = 42 x 10^-5 x 10⁶

= 420

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Answer 2

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