BIO340 Recitation 5 Spring 2020 Your professor is having you look at two genes in Drosophila...
BIO340 Recitation 5 Spring 2020
Your professor is having you look at two genes in Drosophila fruit flies to determine experimentally whether they are linked. One trait is eye color and two alleles are present at the gene: se+ is the wild-type allele for red eyes, and se is the mutant, recessive allele for sepia eyes. The second trait is body color and two alleles are present at this gene: e+ is the wild-type allele for grey body, and e is the mutant, recessive allele for ebony body. You have crossed a pure-breeding red-eyed, grey body female by a pure-breeding sepia-eyed, ebony body male to generate F1 heterozygotes for a mapping cross. All the F1 offspring have red eyes and grey bodies. You next cross this F1 with pure-breeding sepia-eyed, ebony body flies and the following results were obtained.
165 red eyes, grey body
136 red eyes, ebony body
166 sepia eyes, ebony body
133 sepia eyes, grey body
A. If the genes were linked, which two phenotypic classes would represent the recombinants?
B. Conduct a Chi-Square analysis to determine if these two genes assort independently.
C. If the genes do not assort independently, calculate the map distance between the two genes.
Homework Answers
Answer:
Expected testcross progeny would be 1:1:1:1 ratio according to Mendel experiments.
|
Phenotype |
Observed(O) |
Expected (E) |
O-E |
(O-E)2 |
(O-E)2/E |
|
red eyes, grey body |
165 |
150.000 |
15.00 |
225.00 |
1.500 |
|
red eyes, ebony body |
136 |
150.000 |
-14.00 |
196.00 |
1.307 |
|
sepia eyes, ebony body |
166 |
150.000 |
16.00 |
256.00 |
1.707 |
|
sepia eyes, grey body |
133 |
150.000 |
-17.00 |
289.00 |
1.927 |
|
Total |
600 |
600 |
6.440 |
Chi-square value = 6.44
Degrees of freedom = Number of phenotypes – 1
Df = 4-1=3
Critical value = 7.81
The chi-square value of 6.44 is less than the critical value of 7.81. We can accept the null hypothesis and the two genes are not linked and they assorted independently.
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BIO340 Recitation 5 Spring 2020
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