Under which of the following conditions will transcription of the lac operon occur when the bacteria...
Under which of the following conditions will transcription of the lac operon occur when the bacteria are originally inoculated in a medium containing both glucose and lactose?
a) Lactose present/glucose present
b) Lactose present/glucose used up
c) Lactose absent/glucose present
d) Lactose absent/glucose used up
e) a and b
Homework Answers
Answer is b) It will be only turned on in the condition when glucose is used up and lactose is present.
It is because glucose is the preferred substrate of bacteria for energy, but when glucose is not available or it is used up,the bacteria use the genes of lac operon for lactose metabolism.
If both glucose and lactose is present, bacteria will use glucose and transcription won't take place because RNA polymerase will keep falling off the promoter site.
If both glucose and lactose is absent, then also transcription won't occur.
If lactose is absent, then also transcription won't occur,as there is no lactose to metabolise.
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