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When carrying out a purification of a mixture of proteins using size exclusion chromatography, you observe...

When carrying out a purification of a mixture of proteins using size exclusion chromatography, you observe a peak eluting after the total volume of the column (Vt). a. What could be a possible explanation for this observation? b. What steps could you take to prevent molecules from eluting beyond to total volume (Vt) of the column?

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