Question

The transcript and protein sequences encoding bovine ribonuclease are shown in Appendix
1. Appendix 2 contains the codon table. Using your knowledge gained from the lecture
“Genome Sequencing – Identifying Genes” and the codon table in Appendix 2, identify:
• the initiating AUG coding for the initiating M (methionine) in the protein
sequence. Circle the codon.
• the stop codon. Place a rectangle over the codon.
• the polyadenylation signal. Double underline this sequence.
What is region between the stop codon and polyA tail called?
What is the region upstream of the start codon called?

Appendix 1 Sequence of bovine ribonuclease
Transcript
1 GCAGAAACUG CCUUCUCUCU CUCAGACAUC AAACUAGAGA CCCAGGUUUC UCCAGGGGAG
61 UGCGGUCAUC AUGGCUCUGA AGUCCCUGGU CCUGUUGUCG CUGUUGGUCC UGGUGCUGCU
121 GCUGGUGCGG GUCCAGCCUU CCCUGGGCAA GGAAACUGCA GCAGCCAAGU UUGAGCGGCA
181 GCACAUGGAC UCCAGCACUU CCGCUGCCAG CAGCUCCAAC UACUGUAACC AGAUGAUGAA
241 GAGCCGGAAC CUGACCAAAG AUCGAUGCAA GCCAGUGAAC ACCUUUGUGC ACGAGUCCCU
301 GGCUGAUGUC CAGGCCGUGU GCUCCCAGAA AAAUGUUGCC UGCAAGAAUG GGCAGACCAA
361 UUGCUACCAG AGCUACUCCA CCAUGAGCAU CACCGACUGC CGUGAGACCG GCAGCUCCAA
421 GUACCCCAAC UGUGCCUACA AGACCACCCA GGCGAAUAAA CACAUCAUUG UGGCUUGUGA
481 GGGAAACCCG UACGUGCCAG UCCACUUUGA UGCUUCAGUG UAGGUCUCUA CCUAAGGCCA
541 GAGCAGCAAG AUGCACCACU UCAUCACAAA GGCACCUGCC UCUCCCCUCA UGUUUCCUUG
601 UCCUGGGGGC AAUAGCUCAA GUUAGUUAGG GCUCUUAUCU CUGCGCACCU UACCAGAAAC
661 ACACACACAG GAUUCCCUGG CAUGAAAGCA AUAACUCAAG CUAGUUAAGU CUUCUAUCCA
721 ACCCACACUU GCUCCCCUGG CCUGAGUCUU GCCCCUGGUG GUUUGGGGGG UGAGGAGUGG
781 GUUGUGAGGU GGGACCUGUG UUAACCAAAU CACUGCUUCU UUCAAUAAAC AUACUUGCAA
841 CCACCUGAAA AAAAAAAAAA AAA
Protein
MALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADV
QAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV

Answer 1

the region between stop codon and the polydenation signal is called 3'UTR

the region upstreaam of the start codon is called 5'UTR region