If a plate with a final dilution of 10-7 produces 75 colonies, calculate the number of...
If a plate with a final dilution of 10-7 produces 75 colonies, calculate the number of colony- forming units per milliliter.
Homework Answers
In this the plate of the 10^7 dilution yielded a count of 75 colonies. Then, the number of bacteria in 1 ml of the original sample can be calculated as follows:
Bacteria/ml = (75) x (10^7) = 7.5 × 10^8 or 75,00,00,000.
75,00,00,000 CFU/ml of original sample
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If a plate with a final dilution of 10-7 produces 75 colonies,
calculate the number of...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution...
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution of bacteria. How many CFU per ml of bacteria were in the uridiluted stock? (1 point)
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You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 223 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-7)...
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony Forming unit) CFU/ml= (Number of Colonies|Dilution factor) (Volume of culture plate) Conclusion: In a narrative form Using the 51 (Chapter 3, Cowan lecture book) Inoculation agar, Incubation, Isolation, Inspection, Identification Use the 5 l-s to explain how it was decided that the infectious southe was or was not the cause The number of colonies in first plate is 79. The number of colonies in...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution of bacteria. How many CFU per ml of bacteria were in the uridiluted stock? (1 point)
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
32. Work this dilution problem, solve for the number of colony forming units (cfu) in one mL of the E. coli sample. ml 1 ml. 1ml. 1ml. 1m. 1 ml A 2 a. 6.3 x 10 b. 6.3 x 10 c. 4.43 x 106 9 ml. 9 ml. d. 4.43 x 103 9mL Sample of E. coll 443 63 colonies colonles
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony Forming unit) CFU/ml= (Number of Colonies|Dilution factor) (Volume of culture plate) Conclusion: In a narrative form Using the 51 (Chapter 3, Cowan lecture book) Inoculation agar, Incubation, Isolation, Inspection, Identification Use the 5 l-s to explain how it was decided that the infectious southe was or was not the cause The number of colonies in first plate is 79. The number of colonies in...
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