Ans. Gtp hydrolysis is least required in transcription process. Gtp is required as a building block for RNA synthesis in transcription.
Ans. In the elongation stage of translation gtp hydrolysis is required.
Ans. Microtubules posses End is stabilized with a GTP cap, largest filament with 25 nm diameter
Actin filaments reacts with formin, plays a role in motility, polymerization occurs at + end, motor proteins move along filament
Intermediate filaments do not have polar ends, is strongest of the three filaments
What stages of transcription requires GTP hudrolysis? (Please specify which stages) What stages of translation requires...
4) Studying the motility of the pathogenic bacteria Listeria and Shigella, which cause food poisoning and dysentery, respectively, significantly advanced our understanding of actin polymerization. These bacteria escape immune surveillance by entering one cell’s cytoplasm and then spreading from cell to cell without exposing themselves to the outside environment. They harness actin polymerization to push themselves against the plasma membrane, generating a membrane protrusion that is engulfed by a neighboring cell, giving them direct access to that cell’s cytoplasm. The...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...