If lactose is accumulating outside of E. coli and is unable to enter, which structural gene...
If lactose is accumulating outside of E. coli and is unable to enter, which structural gene of the lac operon is most likely mutated? (d) Select one: a. lacY b. lacI c. lacA d. lacZ e. lacX
Homework Answers
There is defect in gene Lac Y. Option a is correct.
In lac operon consists of 3 structural Gene's.
Lac Z- code for beta galactosidase, cleave lactose
Lac Y- lactose permease, pore formation in wall and allow entry of lactose
Lac A- acetylase
Out of these gene, Lac Y is responsible for transporting lactose in side of cell. so this gene must be mutated , if lactose is accumulated outside the cell.
Add Answer to:
If lactose is accumulating outside of E. coli and is unable to
enter, which structural gene...
A mutant E. coli strain does not express lacZ, even in the presence of lactose. What...
A mutant E. coli strain does not express lacZ, even in the presence of lactose. What is a possible genotype of the cells? Group of answer choices lacI+ lacP+ lacO+ lacZ+ lacY+ lacA+ lacl+ lacP+ lacO+ lacZ+ lacY+ lacA+ lacI- lacP+ lacO- lacZ+ lacY+ lacA- lacI+ lacP+ lacO+ lacZ- lacY- lacA+ lacI- lacP+ lacOc lacZ+ lacY+ lacA+
which operon will make b-galactosidase even if lactose isn't present? p+ o+ lacZ+ lacI- p- Oc...
which operon will make b-galactosidase even if lactose
isn't present?
p+ o+ lacZ+ lacI-
p- Oc lacZ+ lacI-
p+ Oc lacZ- lacI+
p- o+ lacZ+ lacI+
p+ o+ lacZ+ lacI+
Oc = fails to bind R
regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and – designates a deletion of that gene or site; lacO+ means all operators (O1, O2 and O3) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Answer please E. coli expresses a specific gene in the presence of lactose. The expression of...
Answer please
E. coli expresses a specific gene in the presence of lactose. The expression of this gene is under control by the lac operon, a sequence of nucleotides in the DNA which control the lac Operon al DNA ) laci CAP) (o) laczlacY
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts the function of each of the following elements: a. the promoter for LacI (P(I)) b. the LacI gene c. CRP binding site d. the promoter for the lac operon (P(lac)) e. the operator sequence f. a mutation in lacZ that disrupts the coding region but does not disrupt transcription g. a mutation in lacZ that blocks transcription For each of the above mutations, what...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose for allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. ones where proteins bind DNA genes and regulatory sequences och Operator o Lacy o Lacz o Laca promoter MacBook Air * जीप
help please as soon as possible A mutant E. coli strain is found that does not...
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
which operon will make b-galactosidase even if lactose
isn't present?
p+ o+ lacZ+ lacI-
p- Oc lacZ+ lacI-
p+ Oc lacZ- lacI+
p- o+ lacZ+ lacI+
p+ o+ lacZ+ lacI+
Oc = fails to bind R
regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Answer please
E. coli expresses a specific gene in the presence of lactose. The expression of this gene is under control by the lac operon, a sequence of nucleotides in the DNA which control the lac Operon al DNA ) laci CAP) (o) laczlacY
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose for allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. ones where proteins bind DNA genes and regulatory sequences och Operator o Lacy o Lacz o Laca promoter MacBook Air * जीप
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Most questions answered within 3 hours.
-
Consider a 1.0 L buffer containing 0.092 mol L-1 HCOOH and 0.100
mol L-1 HCOO-. What...
asked 1 minute ago -
Koch Realty has owned a vacant land with a FMV of
$775,000 and an adjusted basis...
asked 7 minutes ago -
It is estimated 29% of all adults in United States invest in
stocks and that 85%...
asked 7 minutes ago -
What does a 2-sided p value of 0.04 mean? (I am not asking if it
is...
asked 21 minutes ago -
A parallel-plate capacitor is made from two aluminum-foil
sheets, each 7.8 cmcm wide and 5.1 mmlong....
asked 22 minutes ago -
1. why is toluene a stronger nucleophile than benzene?
2.why is phenol a stronger nucleophile than...
asked 39 minutes ago -
4. How can you solve for the density of the liquid from the
slope? Please show...
asked 39 minutes ago -
when 2053 j of heat is added to 46.3 g of hexane C6H14 the
temperature increases...
asked 1 hour ago -
I need new and unique answers, please. (Use your own words,
don't copy and paste), Please...
asked 1 hour ago -
MCL 445.111 et seq. deals with Home Solicitation Sales.
MCL stands for Michigan Compiled Laws which...
asked 56 minutes ago -
Which of the following items may not create an NOL?
a.
sole proprietorship loss
b.
personal...
asked 1 hour ago -
A hypothetical solution forms between a solid and a liquid. The
values of the thermodynamic quantities...
asked 59 minutes ago