A biochemist prepares a buffer solution to purify a His-tagged protein. The pH meter gives the...
A biochemist prepares a buffer solution to purify a His-tagged protein. The pH meter gives the reading of 6.45(+or-)0.05. Determine the [H+] and its uncertainty. Show all your work.
Homework Answers
The concentration of H + ions is calculated:
[H +] = 10 ^ -pH = 10 ^ -6.45 = 3.5x10 ^ -7 M
Uncertainty is calculated:
U [H +] = 3.5x10 ^ -7 * 6.45 * (0.05 / 6.45) = 1.75x10 ^ -8 M
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A biochemist prepares a buffer solution to purify a His-tagged
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10. How does your calculated value of Ka compare with the standard value of Ka for acetic acid? Discuss why your value may be larger or smaller than the standard value. Caleutats Ka 3.2x 10-5) Cyato-s Learning Objectives: 1. To test the acidic and basic properties of ionic compounds 2....
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paper or a ph meter?
Post-Lab Questions 1. Which pe o ple and proper 2. Gundulille battert data t opport your 3.Whach of your de cord was the desti n g changes 4. You are burde wp of u sodium barbana Na W s for show how t a H. und he whHO from the 5. Cele the expected pH v al the best carbon 6.12 n e d475 perimet...
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