2. Explain why the addition of higher concentrations of substrate to the enzyme has lesser effect.
2. Explain why the addition of higher concentrations of substrate to the enzyme has lesser effect.
( --/ Question 1 What is the incorrect statement about catalysis? The enzyme-substrate complex has lower free energy than the free substrate The stronger the enzyme-substrate interaction, the higher the Km value. At very high substrate concentrations, the reaction rate is independent of the substrate concentration. The enzyme-substrate complex is not the transition-state molecule. The higher the turnover number of a catalyst, the lower the catalyst concentration needed to reach a certain maximum reaction rate.
The graph below shows an enzyme- controlled reaction. The enzyme concentration is kept constant. At concentrations of substrate greater than X, which of the following statements is true? Rate of reaction 0 Concentration of substrate X The rate of reaction is limited by enzyme concentration. The rate of reaction tends towards zero. The substrate has an inhibitory effect. The products have an inhibitory effect.
During an enzyme-substrate reaction, the concentrations of four (4) components typically change. Draw a clearly labelled graph showing how the concentrations of substrate, product, free enzyme, and enzyme:substrate complex all change over time.
Enzyme X has a Km for substrate A which is twice as large as its Km for substrate B. If the enzyme is added to a solution containing equal, but low concentrations of A and B and Vmax for A and B are equal: substrate A will be used at a rate equal to substrate B substrate A will be used at a rate greater than substrate substrate B will be used at a rate greater than substrate A. substrate...
6 The graph shows the effect of substrate concentration on the rate of an enzyme- controlled reaction. The enzyme concentration is constant. reaction rate Which statement about the graph is correct? A Between Wand X, the substrate concentration number of enzyme molecules is limiting. Between X and Y, the number of enzyme molecules is limiting. Between X and Y, the number of substrate molecules is limiting. Between X and Y, the product concentration remains the same. Thanh in the nammanni...
For an enzyme that follows the Michaelis-Menten kinetic, what substrate concentrations (relative to Km) are needed for the speed of the reaction to be 0.12 there vmax 0.25 there vmax 0.5 there vmax 0.9 there vmax.
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and...
1. The kinetics of an enzyme was examined at various substrate concentrations in both the presence and absence of 3 mM inhibitor Z. The initial velocity data obtained are shown below: [S] (mmoles liter) v (mmoles"litermin) no inhibitor inhibitor Z 1.25 1.67 2.50 5.00 10.0 1.72 2.04 2.63 3.33 4.17 0.98 1.17 1.47 1.96 2.38 (4 pts) Estimat e Vmax and Kw in the presence and absence of inhibitor using the Michaelis Menton curve-fitting program on Kaleidagraph (see lab manual)....
In an S_N2 reaction, the effect of doubling both nucleophile and substrate concentrations at the same time is to multiply the reaction rate by: 1/4 1/2 2 4 The chloride that reacts the fastest with NaOH is What is the major product of the reaction? Which energy-reaction coordinate diagram represents tins reaction? The major product of this reaction is Which compound would be most rapidly hydrolyzed by aqueous HCl to give methanol as one of the products? CH_3OCH_2CH_2CH_3 CH_3OCH_2CH_2OCH_3 CH_3OCH_2CH_2OH...
At high (saturating) substrate concentrations, the rate of an enzyme-catalyzed reaction approaches Vmax. How close does the reaction rate actually get to Vmax? Determine how high (i.e. how many times Km) the substrate concentration must be for the reaction rate to be: a. 98% Vmax (show your work) (2) b. 99% Vmax (answer only) (1) c. 99.9% Vmax (answer only) (1)