During an enzyme-substrate reaction, the concentrations of four (4) components typically change. Draw a clearly labelled...
During an enzyme-substrate reaction, the concentrations of four (4) components typically change. Draw a clearly labelled graph showing how the concentrations of substrate, product, free enzyme, and enzyme:substrate complex all change over time.
Homework Answers
THESE ARE THE FEW EASY TO UNDERSTAND DIAGRAMS: ENZYME SUBSTRATE REACTION
1. 
To catalyze a reaction, an enzyme will bind to one or more reactant molecules. these molecules are the enzyme substrates. The part of the enzyme where substrate binds is called the active site.
2. 
3. 
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During an enzyme-substrate reaction, the concentrations of four
(4) components typically change. Draw a clearly labelled...
A 6. During an enzyme controlled reaction a number of changes in conformation occur in the...
A 6. During an enzyme controlled reaction a number of changes in conformation occur in the erzyme. Which row shows the role of different changes in contormation of an enzyme ? sequence of an enzyme controlled reaction induced fit of substrate enzyme/substrate complex product release changes total free helps release temporary helps temporary energy of reaction bonds bond formation helps temporary bond changes total free energy helps release formation of reaction tempcrary bonds helps temporary bond helps release formation reduces...
The role of an enzyme in an enzyme-catalyzed reaction is to: Select one: a. make the...
The role of an enzyme in an enzyme-catalyzed reaction is to: Select one: a. make the free-energy change for the reaction more favorable. b. ensure that the product is more stable than the substrate. c. ensure that all the substrate is converted to product. d. increase the rate at which substrate is converted into product. e. do none of the above. The focus of the online practical lesson was the enzyme chymotrypsin. The catalytic mechanism by which chymotrypsin reactions occur:...
In an S_N2 reaction, the effect of doubling both nucleophile and substrate concentrations at the same...
In an S_N2 reaction, the effect of doubling both nucleophile and substrate concentrations at the same time is to multiply the reaction rate by: 1/4 1/2 2 4 The chloride that reacts the fastest with NaOH is What is the major product of the reaction? Which energy-reaction coordinate diagram represents tins reaction? The major product of this reaction is Which compound would be most rapidly hydrolyzed by aqueous HCl to give methanol as one of the products? CH_3OCH_2CH_2CH_3 CH_3OCH_2CH_2OCH_3 CH_3OCH_2CH_2OH...
Enzyme Kinetics Lab Guide Table 2: Absorbance values per minute for varied substrate concentrations Time (min)...
Enzyme Kinetics Lab Guide Table 2: Absorbance values per minute for varied substrate concentrations Time (min) Tube A (0mM) Tube B (2.0mM) Tube C (5.0mM) Tube D (8.0mM) Tube E (12.0mM) Tube F (20.0mM) 0 0 0.012 0.066 0.066 0.076 0.191 1 0.011 0.045 0.128 0.128 0.164 0.200 2 0.017 0.096 0.190 0.190 0.219 0.241 3 0.009 0.122 0.234 0.234 0.294 0.313 Table 3. Absorbance values versus substrate concentration at a constant time (from Table 2) Concentration (mM) 0 mM...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound most tightly by his engineered enzyme. Which value should he measure and pay attention to? Km KCAT Vo Both A and B kcat, [S], and [E]t Rodrigo's enzyme was assayed with three substrates (A, B, and C). The SAME enzyme concentration used was for each of the three reactions. The results of the three experiments are plotted as Lineweaver-Burke plots below. Considering what you...
Figure 24 shows how the concentration of substrates and products of a reaction changed over time...
Figure 24 shows how the concentration of substrates and products of a reaction changed over time after the enzyme and the substrate were mixed together. 0.5 Figure 24 moldm 0.4 0.3 concentration of substances 0.2 0.1 0 Deduce which curve shows the concentration of substrate and explain your answer [1] Using the data in the graph, deduce how the activity of the enzyme changed during the experiment. [2] Explain the change in enzyme activity during the experiment. [4m] 10 10
Calculate the change in Gibbs Free Energy (AG) for the following system. Is this reaction endergonic...
Calculate the change in Gibbs Free Energy (AG) for the following system. Is this reaction endergonic or exergonic? For the following reaction (synthesis of aspirin. C_5H_3O_4), it was experimentally determined that Ea = 50 kJ under standard conditions. After the addition of an enzyme, it was experimentally determined that E_aa = 20 kJ: Complete the following graph showing the reaction before (solid line) and after (dashed line) the addition of the enzyme. Label the horizontal lines for the products and...
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be...
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab exercise 1) Suppose that the diagram below represents what occurs during a chemical reaction when an enzyme is present (a) Which letter points to the product? (b) Which letter points to the reactant? (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note:...
Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
5. Consider the generic reaction shown below in which reactant A is converted to product B....
5. Consider the generic reaction shown below in which reactant A is converted to product B. A pure sample of reactant A is allowed to reach equilibrium. The concentrations of both A and B were monitored during this process and plotted below as a function of time. A(8)=B (8) concentration time a. Based on these data, is the value of Key for this reaction greater than or less than 1? Briefly explain how you can tell. b. The reaction is...
A 6. During an enzyme controlled reaction a number of changes in conformation occur in the erzyme. Which row shows the role of different changes in contormation of an enzyme ? sequence of an enzyme controlled reaction induced fit of substrate enzyme/substrate complex product release changes total free helps release temporary helps temporary energy of reaction bonds bond formation helps temporary bond changes total free energy helps release formation of reaction tempcrary bonds helps temporary bond helps release formation reduces...
The role of an enzyme in an enzyme-catalyzed reaction is to: Select one: a. make the free-energy change for the reaction more favorable. b. ensure that the product is more stable than the substrate. c. ensure that all the substrate is converted to product. d. increase the rate at which substrate is converted into product. e. do none of the above. The focus of the online practical lesson was the enzyme chymotrypsin. The catalytic mechanism by which chymotrypsin reactions occur:...
In an S_N2 reaction, the effect of doubling both nucleophile and substrate concentrations at the same time is to multiply the reaction rate by: 1/4 1/2 2 4 The chloride that reacts the fastest with NaOH is What is the major product of the reaction? Which energy-reaction coordinate diagram represents tins reaction? The major product of this reaction is Which compound would be most rapidly hydrolyzed by aqueous HCl to give methanol as one of the products? CH_3OCH_2CH_2CH_3 CH_3OCH_2CH_2OCH_3 CH_3OCH_2CH_2OH...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound most tightly by his engineered enzyme. Which value should he measure and pay attention to? Km KCAT Vo Both A and B kcat, [S], and [E]t Rodrigo's enzyme was assayed with three substrates (A, B, and C). The SAME enzyme concentration used was for each of the three reactions. The results of the three experiments are plotted as Lineweaver-Burke plots below. Considering what you...
Figure 24 shows how the concentration of substrates and products of a reaction changed over time after the enzyme and the substrate were mixed together. 0.5 Figure 24 moldm 0.4 0.3 concentration of substances 0.2 0.1 0 Deduce which curve shows the concentration of substrate and explain your answer [1] Using the data in the graph, deduce how the activity of the enzyme changed during the experiment. [2] Explain the change in enzyme activity during the experiment. [4m] 10 10
Calculate the change in Gibbs Free Energy (AG) for the following system. Is this reaction endergonic or exergonic? For the following reaction (synthesis of aspirin. C_5H_3O_4), it was experimentally determined that Ea = 50 kJ under standard conditions. After the addition of an enzyme, it was experimentally determined that E_aa = 20 kJ: Complete the following graph showing the reaction before (solid line) and after (dashed line) the addition of the enzyme. Label the horizontal lines for the products and...
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab exercise 1) Suppose that the diagram below represents what occurs during a chemical reaction when an enzyme is present (a) Which letter points to the product? (b) Which letter points to the reactant? (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note:...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
5. Consider the generic reaction shown below in which reactant A is converted to product B. A pure sample of reactant A is allowed to reach equilibrium. The concentrations of both A and B were monitored during this process and plotted below as a function of time. A(8)=B (8) concentration time a. Based on these data, is the value of Key for this reaction greater than or less than 1? Briefly explain how you can tell. b. The reaction is...
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