1. Define selective media. 2. Define differential media. 3. Name a media that is selective and...
1. Define selective media.
2. Define differential media.
3. Name a media that is selective and differential and how you can
interpret results as being such.
4. What makes MSA selective? EMB? MAC?
5. What does a yellow color on MSA indicate SPECIFICALLY!!?? (not
name of bacteria)
6. What characteristic is detected by EMB/MAC?
7. What is a positive result on EMB?
8. What is a positive result on MAC?
Homework Answers
1- selective media
contain susbtance that inhibit all but a few type of bacteria and facilitate the isolation of a particular species. These media are used to isolate a particular bacteria specimen where mixed bacterial flora is expected.
2- diffrential media - when media contain subastancw differing characterstic of bacteria it is called differetial media .
Add Answer to:
1. Define selective media.
2. Define differential media.
3. Name a media that is selective and...
Results (EXPECTED results only): Table 1. Expected appearance of microorganisms on selective and/or differential media (Complete...
Results (EXPECTED results only): Table 1. Expected appearance of microorganisms on selective and/or differential media (Complete table and caption, include Growth/No Growth and Color Change/No Color Change). "Include in the caption what it means for there to be growth and/or color change. Media E. aerogenes P. stuartii S. epidermidis S. cerevisiae E.coli S. aureus NA PDA SDA PEA EMB Mac MSA Caption: Fazil
differential, selective, hemolysis, primary media - 12. For each media, identify the following: a. Name of...
differential, selective, hemolysis, primary media
- 12. For each media, identify the following: a. Name of media b. Type of media: general, enriched, selective, differential. Describe what makes it that way. c. Explain how the results are interpreted. d. Interpret the results of each microorganism. Explain how you made each interpretation
Talk specifically about the differential and selective media( MSA and EMB) and also address TSA which...
Talk specifically about the differential and selective media( MSA and EMB) and also address TSA which would be used as a control. You should discuss how they work, what they assess for: both cellular characteristics and metabolic characteristics. Including figures of pathways.
Background Ques part 1) Selective and differential media; EMB and Tsl agar bacill inhabit a wide...
Background
Ques part 1)
Selective and differential media; EMB and Tsl agar bacill inhabit a wide range of environments. Many are significant pathogens, such a coli, Salmonella, Shigella, but many compose our normal flora and inhabit soil and water. Many gra negative bacilli that inhabit our large intestine are called enterics. Enteric pathogens are the most frequent cause of diarrheal illnesses and have become significant contaminants spread through foo Some can also cause respiratory and urinary infections. Experiment 1: EMB...
Mailings 15.15. Differential and selective media lab (2) (Compatibility Mode] Review View . 3 3 +...
Mailings 15.15. Differential and selective media lab (2) (Compatibility Mode] Review View . 3 3 + 1 AaBbCcDdEe AaBbCcDdEe AaBb CD AaBb CcDdl Aa ER No Spacing Heading 1 Heading 2 Normal Inhibitors Name of agar Phenylethyl alcohol Agar (PEA) Substrate None (not a differential media) Polyphenyl alcohol - affects cell membrane and DNA synthesis Indicators Notes/ expected results None (not a differential Inhibits many Gram media) negatives and some gram positives. Used to select for streptococci, enterococci, and staphylococci...
Multiple Choice. Highlight the single correct answer choice. 1. Streak plates are useful in microbiology to...
Multiple Choice. Highlight the single correct answer choice. 1. Streak plates are useful in microbiology to __________. quantify the number of bacteria measure turbidity identify bacteria determine cell shape 2. In the streak-plate technique, the intent is to isolate bacteria by dilution in theory by __________. dilution on a solid surface separating cells within the solid surface using a pipette dilution in water blanks 3. A pure culture consists of which of the following? one genus of microbe one species...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such as A. to inhibit motility B. to stain microbes C to kill bacteria D. to see microbes to detect pH changes due to metabolism What is the purpose of differential medias to encourage growth of the desired bacteria B to isolate few bacteria to observe metabolism of the bacteria D. to stain bacteria E to determine motility 10 Why does Staphylococcus aureus turn yellow...
Eosin Methylene Blue (EMB) Ex. 4.6 16. What is the selective agent in this test? What...
Eosin Methylene Blue (EMB) Ex. 4.6 16. What is the selective agent in this test? What is the differential agent in this test? 17. E. Coli produces a green metallic sheen when grown on an EMB plate. Why is this? If a pink color is produced by bacteria grown on this plate, what does that indicate? Phenol Red Broth Ex. 5.3 18. What purpose did the uninoculated controls serve in this test? After inoculation, what does a yellow result mean?...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
Selective media 1. All organisms that can grow in the presence of the inhibitory molecule in...
Selective media 1. All organisms that can grow in the presence of the inhibitory molecule in MSA are 2. What group of organisms can not grow in the presence of MSA? 3. What compound(s) or molecule(s) are the selective portion of the test called MacConkey? 4. What causes MacConkey to be differential? 5. What does MacConkey differentiate between?
Results (EXPECTED results only): Table 1. Expected appearance of microorganisms on selective and/or differential media (Complete table and caption, include Growth/No Growth and Color Change/No Color Change). "Include in the caption what it means for there to be growth and/or color change. Media E. aerogenes P. stuartii S. epidermidis S. cerevisiae E.coli S. aureus NA PDA SDA PEA EMB Mac MSA Caption: Fazil
differential, selective, hemolysis, primary media
- 12. For each media, identify the following: a. Name of media b. Type of media: general, enriched, selective, differential. Describe what makes it that way. c. Explain how the results are interpreted. d. Interpret the results of each microorganism. Explain how you made each interpretation
Background
Ques part 1)
Selective and differential media; EMB and Tsl agar bacill inhabit a wide range of environments. Many are significant pathogens, such a coli, Salmonella, Shigella, but many compose our normal flora and inhabit soil and water. Many gra negative bacilli that inhabit our large intestine are called enterics. Enteric pathogens are the most frequent cause of diarrheal illnesses and have become significant contaminants spread through foo Some can also cause respiratory and urinary infections. Experiment 1: EMB...
Mailings 15.15. Differential and selective media lab (2) (Compatibility Mode] Review View . 3 3 + 1 AaBbCcDdEe AaBbCcDdEe AaBb CD AaBb CcDdl Aa ER No Spacing Heading 1 Heading 2 Normal Inhibitors Name of agar Phenylethyl alcohol Agar (PEA) Substrate None (not a differential media) Polyphenyl alcohol - affects cell membrane and DNA synthesis Indicators Notes/ expected results None (not a differential Inhibits many Gram media) negatives and some gram positives. Used to select for streptococci, enterococci, and staphylococci...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such as A. to inhibit motility B. to stain microbes C to kill bacteria D. to see microbes to detect pH changes due to metabolism What is the purpose of differential medias to encourage growth of the desired bacteria B to isolate few bacteria to observe metabolism of the bacteria D. to stain bacteria E to determine motility 10 Why does Staphylococcus aureus turn yellow...
Eosin Methylene Blue (EMB) Ex. 4.6 16. What is the selective agent in this test? What is the differential agent in this test? 17. E. Coli produces a green metallic sheen when grown on an EMB plate. Why is this? If a pink color is produced by bacteria grown on this plate, what does that indicate? Phenol Red Broth Ex. 5.3 18. What purpose did the uninoculated controls serve in this test? After inoculation, what does a yellow result mean?...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
Selective media 1. All organisms that can grow in the presence of the inhibitory molecule in MSA are 2. What group of organisms can not grow in the presence of MSA? 3. What compound(s) or molecule(s) are the selective portion of the test called MacConkey? 4. What causes MacConkey to be differential? 5. What does MacConkey differentiate between?
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