Why do biochemists use E^1% values over molar extinction coefficients? I know this has to do...
Why do biochemists use E^1% values over molar extinction coefficients?
I know this has to do with the fact it is super hard to obtain 1 M solution of a protein in a lab. But why?
We are currently doing spectrophotometry in my biochemistry lab.
Homework Answers
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Why do biochemists use E^1% values over molar extinction
coefficients?
I know this has to do...
How do I convert Extinction coefficients of (48853 M^-1 cm^-1) to (mg/ml)^-1 cm^-1 or (ml/mg)*cm^-1
How do I convert Extinction coefficients of (48853 M^-1 cm^-1) to (mg/ml)^-1 cm^-1 or (ml/mg)*cm^-1
I am doing a lab on the determination of the equilibrium constant for the formation of...
I am doing a lab on the determination of the equilibrium constant for the formation of Fe(SCN)+2. We used a spectrometer to measure the absorption of solutions with a varying initial concentration of Fe+3, but constant concentrations of SCN-. The reaction equation of Fe+3 + SCN- —> Fe(SCN)+2. Along with the SCN- and Fe+3 stock solutions, we also combined them with .3 M HNO3. We then calculated an average molar absorptivity and the equilibrium concentration of Fe(SCN)+2. From that concentration...
Percent by mass of dye in Fruit Loops cereal 11.56*100 QUESTIONS: 1. If the molar extinction coefficient (E) of a...
Percent by mass of dye in Fruit Loops cereal 11.56*100 QUESTIONS: 1. If the molar extinction coefficient (E) of a compound is 2.5 x 108 moli'cmat $28 nm and the Abs Cat 528 nm) 0.254 Dind the concentration of that solution. (assume a path length of 1.0 cm) 2. If a student measures a sample that gives an Abs = 1.85 at the 2max, is this data reliable? If not, then what might they do to improve their data? 3....
Please explain! I know the answers already but I do not understand how to find them....
Please explain! I know the answers already but I do not
understand how to find them.
H3 ОН (10) O(2) Question 2 (24 pts) This small protein has just the 6 ionizable side chains together with N and C termini (pK values in parentheses). 0.1 moles of the protein, in the ionic form shown, is dissolved in 1 L of pure water ("the(9H3N original solution"). Please answer the following questions COO a) what is the approximate pH of the original...
1. Figure I shows an SDS-PAGE gel. A) Rank the 3 proteins by size, from largest...
1. Figure I shows an SDS-PAGE gel. A) Rank the 3 proteins by size, from largest to smallest. Explain why this trend is observed in SDS-PAGE gels. B) What is the purpose of SDS in SDS-PAGE? C) Sample L is the ladder. What is its purpose? D) Typically, PA (polyacrylamide) is used as the gel for protein electrophoresis, whereas agarose is used for DNA electrophoresis. Explain why a different gel material is used, Specifically referring to the pore size of...
Above is my table with my data for the Standing Waves Lab. I have questions as I attempt to do lab report. 1. Table 2 q...
Above is my table with my data for the Standing Waves Lab. I have
questions as I attempt to do lab report.
1. Table 2 question:
What is , for each of the
trials? Is it certain values from my data table? Is it calculated
in some way? How do I get that value?
2. I am presented with this question:
Calculate the experimental value of the frequency
(f ), and find the average
value of the experimental frequency (f...
PROBLEM: Predict the molar absorptivity value of copper sulfate pentahydrate at 380 nm. Here's what I...
PROBLEM: Predict the molar absorptivity value of copper
sulfate pentahydrate at 380 nm.
Here's what I think I have to do:
I think I need to use beer's law. Abs=ebc; e=Abs/bc.
b is 1.66 cm. I think c is .400 M NiSO4, but I'm not sure. I
also think Abs is 0.758 given the graph. (If wavelength is 380 nm,
then it should be between 0.598 and 0.798, right?) My answer is
1.14 L/mol*cm, but not sure if I am...
Hi all the answers are given on this worksheet I just need to know how to do part (e) and calcula...
Hi all the answers are given on this worksheet I just need to
know how to do part (e) and calculate the two numbers in my TI-84.
(I use my TI-84 to get z and p-value)
Problem 4: Two Proportions In 1995, the American Cancer Society randomly sampled 1500 adults of which 555 smoked. In 2005, they surveyed 1730 adults and ound that 578 of them smoked. At the 5% level of significance test the claim that the proportion of...
How do I interpret the p-values in terms of rejecting or failing to reject H0 at...
How do I interpret the p-values in terms of rejecting or failing
to reject H0 at a 95% confidence level? What does the intercept
column mean in terms of p-value? How does the p-value of the F test
compare and what does it mean? In the simple linear regression I'd
conclude age isn't related to pulmonary disease (what does
intercept p-value mean) but for the multiple regression I'd say age
and height aren't related to pulmonary disease but smoking is...
Could you please explain the step 1 and step 2 for me? I know the notes...
Could you please explain the step 1 and step 2 for me? I know
the notes want to show the formula for E(SSTR), but I don't know
what are these two steps doing.
1.2 Expectations of sums of squares Expectation of the sums of squares be derived from the following fact about sample variance. If X1, . . . , Xn are iid, with mean μ and variance σ2, then The sample variance is an unbiased estimator of the variance...
Percent by mass of dye in Fruit Loops cereal 11.56*100 QUESTIONS: 1. If the molar extinction coefficient (E) of a compound is 2.5 x 108 moli'cmat $28 nm and the Abs Cat 528 nm) 0.254 Dind the concentration of that solution. (assume a path length of 1.0 cm) 2. If a student measures a sample that gives an Abs = 1.85 at the 2max, is this data reliable? If not, then what might they do to improve their data? 3....
Please explain! I know the answers already but I do not
understand how to find them.
H3 ОН (10) O(2) Question 2 (24 pts) This small protein has just the 6 ionizable side chains together with N and C termini (pK values in parentheses). 0.1 moles of the protein, in the ionic form shown, is dissolved in 1 L of pure water ("the(9H3N original solution"). Please answer the following questions COO a) what is the approximate pH of the original...
1. Figure I shows an SDS-PAGE gel. A) Rank the 3 proteins by size, from largest to smallest. Explain why this trend is observed in SDS-PAGE gels. B) What is the purpose of SDS in SDS-PAGE? C) Sample L is the ladder. What is its purpose? D) Typically, PA (polyacrylamide) is used as the gel for protein electrophoresis, whereas agarose is used for DNA electrophoresis. Explain why a different gel material is used, Specifically referring to the pore size of...
Above is my table with my data for the Standing Waves Lab. I have
questions as I attempt to do lab report.
1. Table 2 question:
What is , for each of the
trials? Is it certain values from my data table? Is it calculated
in some way? How do I get that value?
2. I am presented with this question:
Calculate the experimental value of the frequency
(f ), and find the average
value of the experimental frequency (f...
PROBLEM: Predict the molar absorptivity value of copper
sulfate pentahydrate at 380 nm.
Here's what I think I have to do:
I think I need to use beer's law. Abs=ebc; e=Abs/bc.
b is 1.66 cm. I think c is .400 M NiSO4, but I'm not sure. I
also think Abs is 0.758 given the graph. (If wavelength is 380 nm,
then it should be between 0.598 and 0.798, right?) My answer is
1.14 L/mol*cm, but not sure if I am...
Hi all the answers are given on this worksheet I just need to
know how to do part (e) and calculate the two numbers in my TI-84.
(I use my TI-84 to get z and p-value)
Problem 4: Two Proportions In 1995, the American Cancer Society randomly sampled 1500 adults of which 555 smoked. In 2005, they surveyed 1730 adults and ound that 578 of them smoked. At the 5% level of significance test the claim that the proportion of...
How do I interpret the p-values in terms of rejecting or failing
to reject H0 at a 95% confidence level? What does the intercept
column mean in terms of p-value? How does the p-value of the F test
compare and what does it mean? In the simple linear regression I'd
conclude age isn't related to pulmonary disease (what does
intercept p-value mean) but for the multiple regression I'd say age
and height aren't related to pulmonary disease but smoking is...
Could you please explain the step 1 and step 2 for me? I know
the notes want to show the formula for E(SSTR), but I don't know
what are these two steps doing.
1.2 Expectations of sums of squares Expectation of the sums of squares be derived from the following fact about sample variance. If X1, . . . , Xn are iid, with mean μ and variance σ2, then The sample variance is an unbiased estimator of the variance...
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