How do I convert Extinction coefficients of (48853 M^-1 cm^-1) to (mg/ml)^-1 cm^-1 or (ml/mg)*cm^-1
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How do I convert Extinction coefficients of (48853 M^-1 cm^-1) to (mg/ml)^-1 cm^-1 or (ml/mg)*cm^-1
QUESTION 8 Calculate the extinction coefficient in (mg/mL) cm from the following data. It is suggested that you use Excel to do this. Give your answer in 3 significant figures. Assume the path length is 1 cm. (You may want to look at the write-up for LAB 19 -1 c (mg/mL) Abs (e 500 nm) 0.001 0.245 0.002 0.490 0.003 0.735 0.004 0.980 0.005 1.100 0.006 1.200 0.007 1.250
Why do biochemists use E^1% values over molar extinction coefficients? I know this has to do with the fact it is super hard to obtain 1 M solution of a protein in a lab. But why? We are currently doing spectrophotometry in my biochemistry lab.
Given the fact that the molar extinction coefficient at 470 nm is 6800 cm-1 M-1 and the radius of the 4-dram vial is 1.00 cm, what would be the absorbance for the highest standard solution (25.00 mL) and for the lowest standard (5.00 mL)? Preparation of iron standard solution (0.01 mg/mL): Weigh (to the nearest 0.1 mg) 0.0350 g of reagent grade (NH4)2Fe(SO4)2•6 H2O into a 500-mL volumetric flask. Dissolve in 250 mL of water that contains ~1 mL of...
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Convert 19.0 m2 to liters. Note that 1 mL = 1 cm 19.0 m 3 = Acetone is a common solvent that has a density of 0.7899 g/mL.. What volume of acetone, in milliliters, has a mass of 15.4 g? volume mL TOOLS x10
(7) Question 1 Convert 1.5 kg to mg: 1- 1500 mg 2- 2,900,000 mg 3- 1.5 x 10^6 mg 4- 1.5 mg Question 2 Convert 6.00 ft to cm: 1- 28.35 cm 2- 183 cm 3- 0.197 cm 4- 1.27 cm Question 3 Convert 3.87 gallons to L, if there are 3.79 L in one gallon? Question 4 Convert 4.57 in to nm, if there are 2.54 cm in one inch. Enter the whole number (do not use scientific notation....
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Please answer both 1. Often when handling proteins, we use concentration in terms of mg/ml. A high concentration is greater than 1 mg/ml and low concentrations are below 0.1 mg/ml (roughly). If you obtained a sample of MDH that was 10 micromoles/L, what is the concentration in mg/ml? Assume that the molecular weight for MDH is 32 kDa. 2. Using Beer's Law, determine the concentration of your sample if the UV-vis reading at 280 nm was 1.2 absorbance units and...