Calculate the binding affinity of a ligand for its receptor from the following data: [Free ligand]...
Calculate the binding affinity of a ligand for its receptor from the following data:
| [Free ligand] (mM) | [Bound Ligand] (mM) |
|
.6 |
2 |
| 1.5 | 4 |
| 3.0 | 6 |
| 6.0 | 8 |
| 15.0 | 10 |
Homework Answers
As we know, Binding Affinity = Constant/ rate constant for backwards reaction
for the above table Binding Affinity will be 0.6/(2-1) = 0.6
1.5/(4-1) = 0.5
3.0/(6-1) = 0.6
6.0/(8-1) = 0.86
15.0/(10-1) = 1.67
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