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what would be the net charge of a protein sequence methionine- cysteine- glutamine-leucine-valine- proline- glutamate- asparagine-...

what would be the net charge of a protein sequence methionine- cysteine- glutamine-leucine-valine- proline- glutamate- asparagine- lysine at ph 1.5, 7 and 12

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Amino acids pKa charge at pH 1.5 charge at pH7 charge at pH12
Methionine 9.21 + - -
Cysteine 10.78 + - -
Glutamine 9.13 + - -
Leucine 9.60 + - -
Valine 9.62 + - -
Proline 10.60 + - -
Glutamate 9.67 + - -
Asparagine 8.84 + - -
Lysine 8.95 + - -
Net charge + - -

If the pH is higher than the pKa value, then charge becomes negative. If the pH is lower than the pKa, then the amino acid will have a positive charge. Therefore the bet charge of protein sequence at pH 1.5 is positive and at pH7 and 12, it is negative.

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