ZuoTi.Pro
Question

Quantitative RT-PCR or RT-qPCR is a highly efficient means of quantifying the levels of individual transcripts....

Quantitative RT-PCR or RT-qPCR is a highly efficient means of quantifying the levels of individual transcripts. Which of the following primers is critical to carry out the first step in RT-qPCR?

-dGGGGGG (poly dG)

-dCCCCCC (poly dC)

-TTTTTTT (poly T)

-dAAAAAA (poly dA)

-none of them

science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

TTTTTTT (POLY T)

Advantages Disadvantages
One-step
  • Less experimental variation since both reactions take place in the same tube
  • Fewer pipetting steps reduces risk of contamination
  • Suitable for high throughput amplification/screening
  • Fast and highly reproducible
  • Impossible to optimize the two reactions separately
  • Less sensitive than two-step because the reaction conditions are a compromise between the two combined reactions
  • Detection of fewer targets per sample
0 0
Add a comment
Know the answer?
Add Answer to:
Quantitative RT-PCR or RT-qPCR is a highly efficient means of quantifying the levels of individual transcripts....
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • 10. The Beck & Watson article is a Group of answer choices quantitative study qualitative study...

    10. The Beck & Watson article is a Group of answer choices quantitative study qualitative study 11. Beck & Watson examined participants' experiences and perceptions using what type of research design? Group of answer choices particpant obersvation phenomenology 12. Select the participants in the Beck & Watson study Group of answer choices Caucasian women with 2-4 children Caucasian pregnant women 13. In the Beck & Watson study, data was collected via a(n) Group of answer choices internet study focus group...

  • urgent need asap 2-4 sentences summarizing the article 4 quotes and in each quote i need...

    urgent need asap 2-4 sentences summarizing the article 4 quotes and in each quote i need 4 bullet points explaning the uote The Nature of Creativity Robert J. Sternberg The field of creativity as it exists today emerged largely as a result of the pioneering efforts of J. P. Guilford (1950) and E. Paul Torrance (1962, 1974). It is wholly fitting to dedicate a special issue of the Creativity Re- search Journal to Torrance because of his seminal con- tributions...

  • second attempt. need asap please 2-4 sentences summarizing the article 4 interesting quotes from the article...

    second attempt. need asap please 2-4 sentences summarizing the article 4 interesting quotes from the article and 4 points explaning for each quote The Nature of Creativity Robert J. Sternberg The field of creativity as it exists today emerged largely as a result of the pioneering efforts of J. P. Guilford (1950) and E. Paul Torrance (1962, 1974). It is wholly fitting to dedicate a special issue of the Creativity Re- search Journal to Torrance because of his seminal con-...

  • How can we assess whether a project is a success or a failure? This case presents...

    How can we assess whether a project is a success or a failure? This case presents two phases of a large business transformation project involving the implementation of an ERP system with the aim of creating an integrated company. The case illustrates some of the challenges associated with integration. It also presents the obstacles facing companies that undertake projects involving large information technology projects. Bombardier and Its Environment Joseph-Armand Bombardier was 15 years old when he built his first snowmobile...

  • what discuss can you make about medicalization and chronic disease and illness? Adult Lealth Nursing Ethics...

    what discuss can you make about medicalization and chronic disease and illness? Adult Lealth Nursing Ethics mie B. Butts OBJECTIVES After reading this chapter, the reader should be able to do the following: 1. Explore the concept of medicalization as it relates to the societal shift away from physician predominance of the 1970s. 2. Differentiate among the following terms: compliance, noncompliance, adherence, nonadherence, and concordance. 3. Examine cultural views with regard to self-determination, decision making, and American healthcare professionals' values...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT