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At a pH of 7.0 predict what will happen to vasopressin (in its uncapped/free terminal amine,...

At a pH of 7.0 predict what will happen to vasopressin (in its uncapped/free terminal amine, but oxidized form) if...

a) you applied a cell lysate containing vasopressin to an anion exchange column (i.e. would it stick)? How might you exploit this column (e.g. by adjusting pH, e.g. pH 4, pH 11, and running specimen over the column again) to increase its purity?

b) Predict what would happen to vasopressin if you attempted to purify it from your lysate using a standard protein SDS-PAGE approach, using 7% acrylamide to polymerize. How might you improve purification of the peptide vasopressin using SDS-PAGE?

c) What would be the simplest, quickest way to separate vasopressin from larger proteins present in the cell lysate (single, quick step)? Explain the method and what materials/devices you would need.

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