You made the following buffer in the lab: 0.600 M Tris-HCl Buffer, pH 8.4. What will...
You made the following buffer in the lab: 0.600 M Tris-HCl Buffer, pH 8.4. What will the pH of this buffer be at 6 °C°C ?
Homework Answers
pKa changes -0.028 pH units for each degree Celsius when temperature increases. If room temperature is 25 oC but you`ll use it at 50 oC, you will have a decrease of -0.028 x 25 = -0.7 pH units. Prepare your Tris buffer at room temperature adjusting the pH that you want plus 0.7. If you intend to have a pH 8.0 at 50 oC, adjust pH to 8.7.
Similarly you have a solution of pH 8.4 and it must be at room temperature so if you want this at 6oC then -0.028 x 19 = -0.532 and your resultant pH is approx 7.1 at 6oC
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