A certain procedure in lab calls for 400 mL of 0.250 M Tris buffer at pH...
A certain procedure in lab calls for 400 mL of 0.250 M Tris buffer at pH 7.00. You have an available stock bottle of 1.00 M Tris at pH 8.30 and look up the pKa of Tris which is 8.00. You also have stocks of 12.00 M HCl, 12.0 M NaOH, and ddH2O. Which chemicals do you need to add, and what volume? Please do not use ice tables as we are supposed to solve this without them. Thank you!
Homework Answers
Add Answer to:
A certain procedure in lab calls for 400 mL of 0.250 M Tris
buffer at pH...
A certain procedure in lab calls for 400 mL of 0.250 M Tris buffer at pH...
A certain procedure in lab calls for 400 mL of 0.250 M Tris buffer at pH 7.00. You have an available stock bottle of 1.00 M Tris at pH 8.30 and look up the pKa of Tris which is 8.00. You also have stocks of 12.00 M HCl, 12.0 M NaOH, and ddH2O. Which chemicals do you need to add, and what volume?
Suppose you want to make 500 mL of a 0.20 M Tris buffer at pH 8.0....
Suppose you want to make 500 mL of a 0.20 M Tris buffer at pH 8.0. On the shelf in lab, you spot a bottle of 1.0 M Tris at pH 6.8 and realize you can start with that to make this new buffer. Assuming you have 5.0 M HCl and 5.0 M NaOH at your disposal, how could you make this 0.20 M Tris buffer at pH 8.0 from the 1.0 M Tris, pH 6.8? (The pKa of Tris...
You made the following buffer in the lab: 0.600 M Tris-HCl Buffer, pH 8.4. What will...
You made the following buffer in the lab: 0.600 M Tris-HCl Buffer, pH 8.4. What will the pH of this buffer be at 6 °C°C ?
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a)...
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the buffer. b) Calculate the mass of Tris base (121.1 MW) to add to the buffer. c) You then measure the pH of the buffer and find it to be 8.2. What amount of 1 M HCl must be added to reach a pH of 7.8? pKa of Tris is 8.06. Ignore volume change...
If you mixed 50 mL of 0.1 M TRIS acid with 60 mL of 0.2 M...
If you mixed 50 mL of 0.1 M TRIS acid with 60 mL of 0.2 M TRIS base, the resulting pH is 8.67. How many total mL of 1 M NaOH could you add to the solution and still have a good buffer (i.e. within one pH unit of the pKa which equals 8.3)?
A 210.0 mL buffer solution is 0.250 M in acetic acid and 0.250 M in sodium...
A 210.0 mL buffer solution is 0.250 M in acetic acid and 0.250 M in sodium acetate. 1) What is the pH after addition of 0.0050 mol of HCL 2) What is the pH after addition of 0.0050 mol of NaOH
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps...
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body. A) You need to prepare a stock solution at pH 7.00 with KH2PO4 and Na2HPO4 (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO4 −2 ] + [H2PO4 − ] = 0.3...
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI...
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI (110 mM, MW = 121.14 g/mol) Urea (0.075 M, 60.06 g/mol) NaClI (150 mM, FW 58.44 g/mol) Dithiothreitol (4 mM, MW = 154.25 g/mol) B-Mercaptoethanol (50 mM, MW 78.13 g/mol) You need to make 2500 mL of this buffer. In your lab you have a bottle of 500 mM Tris-HCl, 125 mM β-mercaptoethanol, and solid urea, NaCl, and dithiothreitol. Calculate the amount of each...
Given stocks of 1 M Lactic Acid (pKa 3.86) at pH 3.5, 2 M naOH, 2...
Given stocks of 1 M Lactic Acid (pKa 3.86) at pH 3.5, 2 M naOH, 2 M HCl and water: how would you prepare 4 liters of 0.05 M Lactic Acid at pH 3.25? Give 2 M Tris, pH 7.1 (pKa 8.0), 5 M NaOH, 5 M HCl, and water: how would you prepare 2 L of 0.2 M Tris, pH 7.4?
1. Calculate the pH of a buffer solution prepared by mixing 0.250 L of 0.150 M...
1. Calculate the pH of a buffer solution prepared by mixing 0.250 L of 0.150 M acetic acid with 0.200 L of 0.250 M sodium acetate. Ka acetic acid = 1.8 x 10-5 Calculate the pH of this solution of after the addition of 0.003 L of 0.200 M HCL 2. Consider a buffer solution prepared by mixing 0.250 L of 0.150 M acetic acid with 0.200 L of 0.250 M sodium acetate. Ka acetic acid = 1.8 x 10-5...
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the buffer. b) Calculate the mass of Tris base (121.1 MW) to add to the buffer. c) You then measure the pH of the buffer and find it to be 8.2. What amount of 1 M HCl must be added to reach a pH of 7.8? pKa of Tris is 8.06. Ignore volume change...
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI (110 mM, MW = 121.14 g/mol) Urea (0.075 M, 60.06 g/mol) NaClI (150 mM, FW 58.44 g/mol) Dithiothreitol (4 mM, MW = 154.25 g/mol) B-Mercaptoethanol (50 mM, MW 78.13 g/mol) You need to make 2500 mL of this buffer. In your lab you have a bottle of 500 mM Tris-HCl, 125 mM β-mercaptoethanol, and solid urea, NaCl, and dithiothreitol. Calculate the amount of each...
Given stocks of 1 M Lactic Acid (pKa 3.86) at pH 3.5, 2 M naOH, 2 M HCl and water: how would you prepare 4 liters of 0.05 M Lactic Acid at pH 3.25? Give 2 M Tris, pH 7.1 (pKa 8.0), 5 M NaOH, 5 M HCl, and water: how would you prepare 2 L of 0.2 M Tris, pH 7.4?
1. Calculate the pH of a buffer solution prepared by mixing 0.250 L of 0.150 M acetic acid with 0.200 L of 0.250 M sodium acetate. Ka acetic acid = 1.8 x 10-5 Calculate the pH of this solution of after the addition of 0.003 L of 0.200 M HCL 2. Consider a buffer solution prepared by mixing 0.250 L of 0.150 M acetic acid with 0.200 L of 0.250 M sodium acetate. Ka acetic acid = 1.8 x 10-5...
Most questions answered within 3 hours.
-
Consider the reaction, C3 H8 + O2 --> CO2 + H2O. How many
moles of O2...
asked 1 hour ago -
You and your opponent both roll a fair die. If you both roll the
same number,...
asked 1 hour ago -
In a study of the accuracy of fast food drive-through orders,
Restaurant A had 257 accurate...
asked 1 hour ago -
Identify and describe in detail the four categories of
institutions that could be included in a...
asked 1 hour ago -
In python
class Customer:
def __init__(self, customer_id, last_name, first_name, phone_number, address):
self._customer_id = int(customer_id)
self._last_name =...
asked 1 hour ago -
What is an example of a limitation in implementing a new
ERP system and how it...
asked 1 hour ago -
In a section of 9.7cm of an artery with a radius of 2.6mm there
is a...
asked 1 hour ago -
the two carboxylic acid groups of aspartic acid have different
acidities with pKa values of 2.1...
asked 1 hour ago -
Would CuCO3 aqueous salt combined with calcium chloride
form a solid precipitate? If so, what would...
asked 1 hour ago -
How do ECM Solutions assist in embedding a culture of continuous
improvement in an organization? (Project...
asked 1 hour ago -
Directions
These directions introduce the idea of Essential Questions.
Since this may be a new concept...
asked 1 hour ago -
1.b. Fiscal policy is said to suffer from ‘crowding out’.
Explain what this means and why...
asked 2 hours ago