Question

How would I prepare both solutions? Calculations needed to see process

* Prepare 100 mL of enzyme invertase solution (0.1 g / L) in the acetates buffer (pH 4.6).

* Prepare solutions of sucrose, in the acetates buffer, with the following concentrations (g / L): 1, 5, 10, 20, 30, 40, 50, 60, 70 and 80.

Answer 1

1)

100ml invertase (0.1g/L) in acetate buffer- pH4.6

- here Molarity of Acetate buffer is not stated therefore we will calculate for standard 0.1Molar.

Weigh 0,406g sodium acetate (anhydrous)

weigh 0.303 g of Acetic acid

Dissolve both in around 70 ml of water

adjust pH to 4.6 using HCl or NaOH

Add 0.01g invertase enzyme

make the volume of all of the above to 100ml with water

2) for 1g/L sucrose- take 1gm sucrose in a container, add a small amount of acetate buffer to dissolve it and make up the volume to 1000ml by using acetate buffer.

for all others, take respective 5gm, 10gm, 20gm, 30gm, 40gm, 50gm, 60gm 70gm, and 80gm, first dissolve each in the small amount of buffer and then make up the volume to 1L using the buffer.