1. Western blotting, ELISA, and IP/Co-IP all manipulate the immune system interaction between antibodies and antigens....
1. Western blotting, ELISA, and IP/Co-IP all manipulate the immune system interaction between antibodies and antigens. Briefly describe this interaction.
2. If you are using ELISA or Western blotting for quantitative analysis, what types of controls might you need?
Homework Answers
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1. Western blotting, ELISA, and IP/Co-IP all manipulate the
immune system interaction between antibodies and antigens....
pls help, ty ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
QUESTION 11 How is the immune system able to recognize a limitless number of different antigens...
QUESTION 11 How is the immune system able to recognize a limitless number of different antigens and epitopes? While each lymphocyte carries receptors that recognize only one type of epitope, the immune system produces a wide variety of lymphocytes each of which carries unique receptors A genetic "memory" of the pathogens your parents encountered (and their parents and so on) is passed on to each generation, increasing the number of possible responses over time. Each lymphocyte is coated with many...
How does the immune system protect us from disease? How do doctors use the immune response...
How does the immune system protect us from disease? How do doctors use the immune response to protect you from disease? How are the antibodies in your body made? How are antibodies that are used in ELISA made? What does ELISA stand for? What enzymes were used in the Labster simulation? Why? Why are enzymes used in this immunoassay? Why do you need to assay positive and negative control samples as well as your experimental samples? If the sample gave...
Question 12 parts a,b,c Q12. Using the information provided below on ELISA, answer the following questions...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
Instructions for Part 2: Answer the following questions: 1. How does the immune system protect us...
Instructions for Part 2: Answer the following questions: 1. How does the immune system protect us from disease? 2. How do doctors use the immune response to protect you from disease? 3. How are the antibodies in your body made? 4. How are antibodies that are used in ELISA made? 5. What does ELISA stand for? 6. What enzymes were used in the Labster simulation? Why? 7. Why are enzymes used in this immunoassay? 8. Why do you need to...
Suppose you are working on a set of experiments using Western blotting. Using an antibody that...
Suppose you are working on a set of experiments using Western blotting. Using an antibody that recognizes amino sequences encoded by exon 3 of a five-exon gene, you have demonstrated that a protein is highly expressed in a particular cell line. To assess the mRNA, you design reverse transcriptase polymerase chain reaction (rtPCR) primers to exons 2 and 4, which flank exon 3. Despite all your efforts, you cannot amplify an mRNA. You know that it is indeed expressed, since...
Briefly describe why an immune precipitation (IP) was used in this figure? Compare lanes 1 and...
Briefly describe why an immune precipitation (IP) was used in
this figure?
Compare lanes 1 and 3?
What question is being asked?
What is the result?
I need help with A and B please. can you explain how you got
that answer? Its for cell and molecular.
Cytosol Anti-Dnak IP 1- Dnak ITIL IT | 1 2 3 4 5 6 wt Adnak wt after 1% SDS Adnak / labeled Adnak +unlabeled wt Figure 1. Transient Interaction of Newly Synthesized...
37 through 42 37. lgG, igA, gM, lgD, and lgE are all examples of a. antigens....
37 through 42
37. lgG, igA, gM, lgD, and lgE are all examples of a. antigens. b. isotypes. c. idiotypes. d. allotypes 38. Using the data from the differential white blood cell count table below, which patient profile displays a parasitic infection? Table 15.1 Guidelines for Interpreting White Blood Cell (WBC) Counts writh Diferential a. Patient 1 b. Patient 3 b. Patient 2 d. None 39, A micr would be expected to occur? obe is ingested and broken into fragments...
please answer all 4 questions asap Question 15 3 pts Several types of innate immune cells...
please answer all 4 questions asap
Question 15 3 pts Several types of innate immune cells attack antigens by: o Promoting apoptosis in cells containing the antigen o Producing antibodies that neutralize the antigen o Scaring them away o Phagocytosis and digestion internally Question 16 3 pts In a primary response, what part of the immune response responds the most quickly to a novel antigen? o Humoral adaptive immunity o Cell-mediated adaptive immunity O Innate immunity Question 17 3 pts...
Not sure how to answer these immunology questions! Help! 1) An otherwise healthy person is involved...
Not sure how to answer these immunology questions! Help! 1) An otherwise healthy person is involved in an accident that requires kidney transplantation within a week. As the attending immunologist you are aware of three family members (no identical twins) that are willing to donate a kidney. What would you do to prepare the patient to minimize rejection?1) Detail three tests you would use to determine your ultimate donor tissue. Outline the rational that you would use to choice your...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
QUESTION 11 How is the immune system able to recognize a limitless number of different antigens and epitopes? While each lymphocyte carries receptors that recognize only one type of epitope, the immune system produces a wide variety of lymphocytes each of which carries unique receptors A genetic "memory" of the pathogens your parents encountered (and their parents and so on) is passed on to each generation, increasing the number of possible responses over time. Each lymphocyte is coated with many...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
Instructions for Part 2: Answer the following questions: 1. How does the immune system protect us from disease? 2. How do doctors use the immune response to protect you from disease? 3. How are the antibodies in your body made? 4. How are antibodies that are used in ELISA made? 5. What does ELISA stand for? 6. What enzymes were used in the Labster simulation? Why? 7. Why are enzymes used in this immunoassay? 8. Why do you need to...
Briefly describe why an immune precipitation (IP) was used in
this figure?
Compare lanes 1 and 3?
What question is being asked?
What is the result?
I need help with A and B please. can you explain how you got
that answer? Its for cell and molecular.
Cytosol Anti-Dnak IP 1- Dnak ITIL IT | 1 2 3 4 5 6 wt Adnak wt after 1% SDS Adnak / labeled Adnak +unlabeled wt Figure 1. Transient Interaction of Newly Synthesized...
37 through 42
37. lgG, igA, gM, lgD, and lgE are all examples of a. antigens. b. isotypes. c. idiotypes. d. allotypes 38. Using the data from the differential white blood cell count table below, which patient profile displays a parasitic infection? Table 15.1 Guidelines for Interpreting White Blood Cell (WBC) Counts writh Diferential a. Patient 1 b. Patient 3 b. Patient 2 d. None 39, A micr would be expected to occur? obe is ingested and broken into fragments...
please answer all 4 questions asap
Question 15 3 pts Several types of innate immune cells attack antigens by: o Promoting apoptosis in cells containing the antigen o Producing antibodies that neutralize the antigen o Scaring them away o Phagocytosis and digestion internally Question 16 3 pts In a primary response, what part of the immune response responds the most quickly to a novel antigen? o Humoral adaptive immunity o Cell-mediated adaptive immunity O Innate immunity Question 17 3 pts...
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