Describe eukaryotic meiotic recombination pathway and compare/contrast with E. coli's homologous recombination.
Eukarotic meiotic recombination at multiple point on almost all chromosomes and its time taking process,occurs at pachytene stage of prophase I of meiosis I. This leads to recombinant gamate formation which leads to much evolved organisms better suits for existing environment.
In case of E.coli homologous recombination is for small noog genes located on haploid single chromosomes. It's fast process. It's done by process transformation, conjugation and transduction which causes the small fragments of gene carried by external source or bacteria or bacteriophage . For short duration i.e during transformation, conjugation or transduction, bacterial chromosome become partially diploid called merozygote.
Hope it's clear.thanks
Describe eukaryotic meiotic recombination pathway and compare/contrast with E. coli's homologous recombination.
Compare and contrast non-homologous end joining and homologous recombination. What are the pros and cons of each?
Describe the following errors of meiotic recombination: A) Unequal crossing over B) Exon shuffling
Describe at least 4 genetic consequences of homologous recombination
Describe 2 meiotic sources of recombination? Why is plasmid based engineered DNA considered recombinant?
Homologous Recombination Lecture Molecular Biology The ability for yeast and E. coli to do homologous recombination easily make it very easy to generate specific mutations. To “knock-out” a gene, you need to transform the cells with a PCR product containing an antibiotic resistance cassette flanked by the genomic DNA sequence where you would like to insert the cassette. Based on our lecture, diagram the process of recombination of the left flank of the following Kanamycin resistance cassette into the E....
1. Homologous recombination can happen between non-identical DNA sequences. T/F? 2. Homologous recombination can happen in_______ a) meiosis b) mitosis c) both 3. Homologous recombination in meiosis has the main purpose of_____ a) DNA repair b) Creating new chromosomes c) Sealing double-stranded breaks 4. Strand invasion usually happens without enzymatic assistance. T/F? 5. When replication fork runs into a nick, it results in a_______ a) single-stranded break b) double-stranded break 6. The invading end is usually a _______ a) 3'...
Compare and contrast Prokaryotic and Eukaryotic DNA replication For exam you must be able to describe at least 3 specific similarities between the two and 3 specific differences between the two. An example of 1 difference is that prokaryotic replication has 1 replication bubble (one oriC). Eukaryotic replication has many replication bubbles (multiple origins of replication)
Describe the fate of each strand of donor dsDNA during homologous recombination
Describe how Nucleotide Excision Repair, Homologous Recombination Repair (HRR), and Non-homologous End Joining (NHEJ) are beneficial for repair of damaged DNA. If you could choose between having HRR and NHEJ performed, which would you choose and why?
You have discovered a special dye that reveals the position of recombination sites on meiotic chromosomes. You use this dye to count the number of recombination sites and then compare this to the number of genetic exchanges (spots that stain with the dye) that you can detect by looking at the segregation of markers across the genome. Therefore, you find many more recombination sites as compared to actual genetic exchanges. Explain this result.