Describe 2 meiotic sources of recombination? Why is plasmid based engineered DNA considered recombinant?
Describe 2 meiotic sources of recombination?
Why is plasmid based engineered DNA considered recombinant?
Homework Answers
Recombination is the process in which two DNA molecules exchange pieces of their genetic material with each other. The best instance of recombination occurs during meiosis 1 ( pachytene stage of prophase 1). The homologous chromosomes line up in pairs and exchange segments of DNA. This process is also called crossing over and results in gametes with new combinations of genes. It increases the genetic diversity in offspring that are produced by the fusion of such gametes during sexual reproduction.
Gene conversion is also a type of recombination. It is a mechanism during which the homologous sequences are made identical. During this process a section of genetic material is usually copied from one chromosome to another one but the donating chromosome does not change .
2). The recombinant DNA is created by combining DNA from two or more sources. This process usually involves combining the DNA of different organisms. The plasmid based engineered DNA is considered recombinant as it has been manipulated to have novel genes or a section of DNA. Researchers can insert new pieces of DNA into these plasmids to create recombinant plasmids. These novel DNA pieces are inserted at restriction sites. These plasmids are then transferred to bacteria by transformation.
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Describe 2 meiotic sources of recombination?
Why is plasmid based engineered DNA considered recombinant?
Sub-cloning is a powerful technique that involves the preparation of a recombinant plasmid containing a DNA...
Sub-cloning is a powerful technique that involves the preparation of a recombinant plasmid containing a DNA fragment of interest and subsequent transformation that plasmid. In this experiment, you preformed a transformation, but the recombinant plasmid containing phage DNA was already prepared. Describe how you would make a recombinant plasmid containing a fragment you have already generated by PCR , including what enzymes you would use and the approximate length of time each step would take.
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid"...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid"...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
CLASS: Cell Biology (Biology 204) TOPIC: Bacterial transformation, purification, and recombination Why does the plasmid DNA...
CLASS: Cell Biology (Biology 204) TOPIC: Bacterial transformation, purification, and recombination Why does the plasmid DNA remain intact while the bacterial chromosome is sheared and subsequently lost during the miniprep procedure?
Based on the following picture: 1- Write the DNA sequence of the recombinant pET/GFP plasmid beginning...
Based on the following picture:
1- Write the DNA sequence of the recombinant pET/GFP plasmid
beginning at the ATG translational start codon and continuing
through the first 4 codons (triplets) of the GFP coding region. Put
a box around the NheI site.1-
2- Also, based on the DNA sequence you have written out above,
write the amino acid sequence of the protein that is encoded by
this DNA. Box the His-tag. Underline the region that corresponds to
the GFP protein...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
Please answer the following parts: A) Will transposition of antibiotic resistance on either plasmid occur as...
Please answer the following parts:
A) Will transposition of antibiotic resistance on either
plasmid occur as deawn? Briefly, why? Or why not?
B) Draw what would result from a single recombination between
the two IS2 sequences. Assume the recombination occurs in the
center of the IS sequences. Make sure you show the locations of amp
and tet on your recombinant, and use a white arrow to represent IS2
from plasmid A and a black arrow for IS2 from plasmid B,...
BTH2732 Recombinant DNA Technology For answer all questions below part, Ignore question 1. Just do question...
BTH2732 Recombinant DNA Technology
For answer all questions below part, Ignore question 1. Just
do question 2 and below
Part A: Refer to Lecture 3 & Supplementary Video: https://www.youtube.com/watch?v SiwNtQYLKeU Virtual cloning of the Human p53 gene You are required to devise a set of practical methodologies in order to carry out a relatively simple molecular biology task. 1. You must identify the steps required 2. Formulate a set of practical protocols needed to carry out those steps . Consider...
1) When/why do cells divide using meiosis? 2) Describe the events in the meiotic cell cycle...
1) When/why do cells divide using meiosis? 2) Describe the events in the meiotic cell cycle including any stages, phases and/or subphases.
1. 2. 13. Which statement explains why the recombination frequency between two genes is always less...
1.
2.
13.
Which statement explains why the recombination frequency between two genes is always less than 50%? Recombination cannot be more than 50% because chromosomes are only 50 map units in length. The genotype of the F, gametes will always be 50% parental gametes and 50% recombinant gametes. Genes with a recombination frequency near 50% are unlinked and have an equal likelihood of being inherited together or separately. F1 gametes always have 50% of their alleles from each parental...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
Based on the following picture:
1- Write the DNA sequence of the recombinant pET/GFP plasmid
beginning at the ATG translational start codon and continuing
through the first 4 codons (triplets) of the GFP coding region. Put
a box around the NheI site.1-
2- Also, based on the DNA sequence you have written out above,
write the amino acid sequence of the protein that is encoded by
this DNA. Box the His-tag. Underline the region that corresponds to
the GFP protein...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
Please answer the following parts:
A) Will transposition of antibiotic resistance on either
plasmid occur as deawn? Briefly, why? Or why not?
B) Draw what would result from a single recombination between
the two IS2 sequences. Assume the recombination occurs in the
center of the IS sequences. Make sure you show the locations of amp
and tet on your recombinant, and use a white arrow to represent IS2
from plasmid A and a black arrow for IS2 from plasmid B,...
BTH2732 Recombinant DNA Technology
For answer all questions below part, Ignore question 1. Just
do question 2 and below
Part A: Refer to Lecture 3 & Supplementary Video: https://www.youtube.com/watch?v SiwNtQYLKeU Virtual cloning of the Human p53 gene You are required to devise a set of practical methodologies in order to carry out a relatively simple molecular biology task. 1. You must identify the steps required 2. Formulate a set of practical protocols needed to carry out those steps . Consider...
1.
2.
13.
Which statement explains why the recombination frequency between two genes is always less than 50%? Recombination cannot be more than 50% because chromosomes are only 50 map units in length. The genotype of the F, gametes will always be 50% parental gametes and 50% recombinant gametes. Genes with a recombination frequency near 50% are unlinked and have an equal likelihood of being inherited together or separately. F1 gametes always have 50% of their alleles from each parental...
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