Question

Please answer the following parts:

A) Will transposition of antibiotic resistance on either plasmid occur as deawn? Briefly, why? Or why not?

B) Draw what would result from a single recombination between the two IS2 sequences. Assume the recombination occurs in the center of the IS sequences. Make sure you show the locations of amp and tet on your recombinant, and use a white arrow to represent IS2 from plasmid A and a black arrow for IS2 from plasmid B, and appropraite solid or dotted lines to represent plasmid DNAs.

C) Based on your drawing in part B, do you think that transposition if amp and/or tet could occur? Briefly, explain how this could or not occur, referring to your diagram above in “B”

1. 3 pts, I each A.B.C). Suppose that there are 2 plasmids as shown below each of which is shown below, with each containing an IS2 insertion sequence. amp IS2

Answer 1

A) Yes, Because both plasmids have the IS2 homologous regions, then a recombinant event can occur in which antibiotic resistance (amp and tet) are transposed to the other plasmid

B) Result of a single recombination event between the two IS2 sequences.

C) Based on the drawing of the part B, we can observe that the transposition occurs in which both antibiotic resistance were transposed to the plasmid