Homework Answers
A. The transposition of antibiotic resistance will occur on either plasmid because during the event of transposition, the resistance genes would be incorporated and that might confer antiobiotic resistance to the bacteria containing the plasmid. This kind of IS mostly carry their own transposase enzymes and confer proper transposition.
B.
C. Transposition of the antibiotics amp/tet may occur together as there is a recombination phenomenon occurring. If the Insertional sequences are recombined, they would include the antibiotics and provide resistance factor
Add Answer to:
1. (3 pts, I each A,B,C). Suppose that there are 2 plasmids as shown below each...
Please answer the following parts: A) Will transposition of antibiotic resistance on either plasmid occur as...
Please answer the following parts:
A) Will transposition of antibiotic resistance on either
plasmid occur as deawn? Briefly, why? Or why not?
B) Draw what would result from a single recombination between
the two IS2 sequences. Assume the recombination occurs in the
center of the IS sequences. Make sure you show the locations of amp
and tet on your recombinant, and use a white arrow to represent IS2
from plasmid A and a black arrow for IS2 from plasmid B,...
2 Addendum 1 to this lab provides information on the location of the EcoRI, and Sspl...
2 Addendum 1 to this lab provides information on the location of the EcoRI, and Sspl cut sites in your BlueScript vector and the 1.3 kb EcoRI fragment. Using this information, (a) draw the two possible restriction maps for the recombinant pBlueKan plasmid that you constructed (see below). There are two possible maps because the 1.3 kb fragment could have been cloned in either orientation relative to the vector, Next, (b) predict the sizes of the EcoRI. and Sspl frements...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Section 1: Short Answer- Briefly answer each question in the space immediately below it S pts ea....
Generic set W,X and Y
Section 1: Short Answer- Briefly answer each question in the space immediately below it S pts ea. Consider generic sets w, x, and y, and also A-u2-1, 6, 9). B-u,-1, 3, 6. c 3(-1.63) . eenerically. what does P(X) represent? Answer Here 2. Generically, what does / y / represent? 3. (15 pts) RE #a1: Then, generically. I y I : what value? 4. (10 pts) Let W P()3 W yl W? Work this out...
2.D use reaction from part A to do D ; and 3 b. Organic chem
2.D use reaction from part A to do D ; and 3 b. Organic
chem
2. a. Predict which of the reactions shown below will ocur at a faster rate: oWg OMe HSO4, HNO, NO2 i tachioninthe pava posto H2SO HNO B What does your answer in part a imply about the major product of the nitration of anisole? Show detailed mechanism(s) for the reactions in part a and give energy diagrams (reaction coordinate diagrams) for the two mechanisms. Take...
1. (2 pts) Homologous chromosomes a. come from different parents b. have the same genes c....
1. (2 pts) Homologous chromosomes a. come from different parents b. have the same genes c. may have different alleles d. have very similar DNA sequences e. all of the above f. none of the above 2. (3 pts) List the three ways that sexual reproduction increases the genetic variability of a population more than does asexual reproduction. 3. (2 pts) An organism has six independent (unlinked) genes and the following genotype: aaBbDdEEffag. How many different gametes could this organism...
Your Name: 1. (a) Discuss with your group why each transformation (A, B, and C) shown...
Your Name: 1. (a) Discuss with your group why each transformation (A, B, and C) shown below will not produce the product shown. Be sure to include any applicable discussion on choices of reagents, stereochemical outcome expected versus what is shown in the product. Your peer review for other students should include discussion about why you agree or disagree with the others' responses. Don't just simply comment "I agree" or I disagree". Discuss helpful and insightful suggestions for responses you...
Suppose there are two firms, 1 and 2, each with M C = AC = 10....
Suppose there are two firms, 1 and 2, each with M C = AC = 10. They each choose quantities of output, y1 and y2. The market demand is p= 70−2y, where y=y1+y2. Each firm discounts the future at rate δ <1per period. a. [6 marks] First calculate the Cournot equilibrium outputs. Call these y∗1 and y∗2. b. [4 marks] Next, calculate how much each firm would produce if the two firms colluded to act as a monopoly. Call these...
looking for question 3 a,b,c,d,e,f,g,h and i. Thank you it's the same question with different situations....
looking for question 3 a,b,c,d,e,f,g,h and i. Thank you
it's the same question with different situations.
3. Now let's use trial-and-error to see if we can figure out the inheritance pattern of this trait. For EVERY pedigree diagram below and on the following pages, label what you know about the genotype of each individual shown from the information provided in the pedigree and the assumption specified above each one. You MUST label ALL FOUR diagrams separately! Genotypes in these pedigrees...
specifically on finite i pmu r the number of objøcts or ways. Leave your answers in fornsiala form, such as C(3, 2) nporkan?(2) Are repeats poasib Two points each imal digits will have at le...
specifically on finite
i pmu r the number of objøcts or ways. Leave your answers in fornsiala form, such as C(3, 2) nporkan?(2) Are repeats poasib Two points each imal digits will have at least one xpeated digin? I. This is the oounting problem Al ancmher so ask yourelr (1) ls onder ipo n How many strings of four bexadeci ) A Compuir Science indtructor has a stack of blue can this i For parts c, d. and e, suppose...
Please answer the following parts:
A) Will transposition of antibiotic resistance on either
plasmid occur as deawn? Briefly, why? Or why not?
B) Draw what would result from a single recombination between
the two IS2 sequences. Assume the recombination occurs in the
center of the IS sequences. Make sure you show the locations of amp
and tet on your recombinant, and use a white arrow to represent IS2
from plasmid A and a black arrow for IS2 from plasmid B,...
2 Addendum 1 to this lab provides information on the location of the EcoRI, and Sspl cut sites in your BlueScript vector and the 1.3 kb EcoRI fragment. Using this information, (a) draw the two possible restriction maps for the recombinant pBlueKan plasmid that you constructed (see below). There are two possible maps because the 1.3 kb fragment could have been cloned in either orientation relative to the vector, Next, (b) predict the sizes of the EcoRI. and Sspl frements...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Generic set W,X and Y
Section 1: Short Answer- Briefly answer each question in the space immediately below it S pts ea. Consider generic sets w, x, and y, and also A-u2-1, 6, 9). B-u,-1, 3, 6. c 3(-1.63) . eenerically. what does P(X) represent? Answer Here 2. Generically, what does / y / represent? 3. (15 pts) RE #a1: Then, generically. I y I : what value? 4. (10 pts) Let W P()3 W yl W? Work this out...
2.D use reaction from part A to do D ; and 3 b. Organic
chem
2. a. Predict which of the reactions shown below will ocur at a faster rate: oWg OMe HSO4, HNO, NO2 i tachioninthe pava posto H2SO HNO B What does your answer in part a imply about the major product of the nitration of anisole? Show detailed mechanism(s) for the reactions in part a and give energy diagrams (reaction coordinate diagrams) for the two mechanisms. Take...
1. (2 pts) Homologous chromosomes a. come from different parents b. have the same genes c. may have different alleles d. have very similar DNA sequences e. all of the above f. none of the above 2. (3 pts) List the three ways that sexual reproduction increases the genetic variability of a population more than does asexual reproduction. 3. (2 pts) An organism has six independent (unlinked) genes and the following genotype: aaBbDdEEffag. How many different gametes could this organism...
Your Name: 1. (a) Discuss with your group why each transformation (A, B, and C) shown below will not produce the product shown. Be sure to include any applicable discussion on choices of reagents, stereochemical outcome expected versus what is shown in the product. Your peer review for other students should include discussion about why you agree or disagree with the others' responses. Don't just simply comment "I agree" or I disagree". Discuss helpful and insightful suggestions for responses you...
looking for question 3 a,b,c,d,e,f,g,h and i. Thank you
it's the same question with different situations.
3. Now let's use trial-and-error to see if we can figure out the inheritance pattern of this trait. For EVERY pedigree diagram below and on the following pages, label what you know about the genotype of each individual shown from the information provided in the pedigree and the assumption specified above each one. You MUST label ALL FOUR diagrams separately! Genotypes in these pedigrees...
specifically on finite
i pmu r the number of objøcts or ways. Leave your answers in fornsiala form, such as C(3, 2) nporkan?(2) Are repeats poasib Two points each imal digits will have at least one xpeated digin? I. This is the oounting problem Al ancmher so ask yourelr (1) ls onder ipo n How many strings of four bexadeci ) A Compuir Science indtructor has a stack of blue can this i For parts c, d. and e, suppose...
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