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CLASS: Cell Biology (Biology 204) TOPIC: Bacterial transformation, purification, and recombination Why does the plasmid DNA...

CLASS: Cell Biology (Biology 204)

TOPIC: Bacterial transformation, purification, and recombination

Why does the plasmid DNA remain intact while the bacterial chromosome is sheared and subsequently lost during the miniprep procedure?

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Answer #1

Plasmid DNA remains intact because in miniprep procedure , NAOH AND SDS Is added and genomic aas well as plasmid DNA both denatured.

Next step is to use potassium acetate to reanneal the separated strands of DNA.

AS genomic DNA is larger than plasmid DNA , genomic DNA can't be reannealed and if reannealed they form tangled with same strand so complementary strands can't reanneal.

Whereas plasmid DNA is short that's why it can be reannealed very easily.

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