CLASS: Cell Biology (Biology 204) TOPIC: Bacterial transformation, purification, and recombination Why does the plasmid DNA...
CLASS: Cell Biology (Biology 204)
TOPIC: Bacterial transformation, purification, and recombination
Why does the plasmid DNA remain intact while the bacterial chromosome is sheared and subsequently lost during the miniprep procedure?
Homework Answers
Plasmid DNA remains intact because in miniprep procedure , NAOH AND SDS Is added and genomic aas well as plasmid DNA both denatured.
Next step is to use potassium acetate to reanneal the separated strands of DNA.
AS genomic DNA is larger than plasmid DNA , genomic DNA can't be reannealed and if reannealed they form tangled with same strand so complementary strands can't reanneal.
Whereas plasmid DNA is short that's why it can be reannealed very easily.
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CLASS: Cell Biology (Biology 204)
TOPIC: Bacterial transformation, purification, and
recombination
Why does the plasmid DNA...
mechanisms of recombination from donor to recipient cell *kind of dna transfered (chromosome, plasmid, or both)...
mechanisms of recombination from donor to recipient cell
*kind of dna transfered (chromosome, plasmid, or both)
REVIEW Transformation Generalized transduction Conjugation F+ A. Kind of DNA transferred (chram oame, plamid or both) Is donor living or dead? Is direct contact required? yes or no Is a virus required? yes or no Will the process occur if DN Aase is in the medium? yes or no
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using a small column to bind DNA molecules during purification of the plasmid DNA. This column binds DNA via ionic interactions. What is the charge on the column which allows it to bind DNA? Answer positive" or "negative and explain your answer. 3. Where is the plasmid at the END of each of the following steps? Circle the letter of the correct answers for each...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
please help and thank you Materials Needed per class: 1 box of 24 microscope slides of...
please help and thank you
Materials Needed per class: 1 box of 24 microscope slides of meiosis 1 1 box of 24 microscope slides of meiosis 2 red and yellow popbead chromosome kits Objectives To become familiar with the process of meiosis and to be able to identify the principal phases of meiosis To understand how the process of meiosis is similar to mitosis and how it differs from mitosis Introduction The genetic information of a cell is encoded in...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during Meiosis I? 26. Explain how these inheritance patterns are considered non-Mendelian. Incomplete Dominance . Multiple Alleles • Codominance X-linked Linkage . Pedigrees - Genetic Disorders 27. What is non-disjunction and how does it affect the chromosome distribution during meiosis? 28. What is a karyotype and what does it allow you to do? 29. Fill in the circles and squares to illustrate the following inheritance...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
mechanisms of recombination from donor to recipient cell
*kind of dna transfered (chromosome, plasmid, or both)
REVIEW Transformation Generalized transduction Conjugation F+ A. Kind of DNA transferred (chram oame, plamid or both) Is donor living or dead? Is direct contact required? yes or no Is a virus required? yes or no Will the process occur if DN Aase is in the medium? yes or no
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using a small column to bind DNA molecules during purification of the plasmid DNA. This column binds DNA via ionic interactions. What is the charge on the column which allows it to bind DNA? Answer positive" or "negative and explain your answer. 3. Where is the plasmid at the END of each of the following steps? Circle the letter of the correct answers for each...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
please help and thank you
Materials Needed per class: 1 box of 24 microscope slides of meiosis 1 1 box of 24 microscope slides of meiosis 2 red and yellow popbead chromosome kits Objectives To become familiar with the process of meiosis and to be able to identify the principal phases of meiosis To understand how the process of meiosis is similar to mitosis and how it differs from mitosis Introduction The genetic information of a cell is encoded in...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during Meiosis I? 26. Explain how these inheritance patterns are considered non-Mendelian. Incomplete Dominance . Multiple Alleles • Codominance X-linked Linkage . Pedigrees - Genetic Disorders 27. What is non-disjunction and how does it affect the chromosome distribution during meiosis? 28. What is a karyotype and what does it allow you to do? 29. Fill in the circles and squares to illustrate the following inheritance...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
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