A. | Transformation | conjugation | generalized transduction |
Kind of DNA transferred | a fragment of linear DNA |
H+- only plasmid can transfer Hfr-both plasmid and chromosomal DNA can transfer, in this process plasmid integrated into the chromosomal DNA then a piece of it transfer into the donor cell. |
generally it occur during the lytic cycle of bacteeiophage. Bacteriophage virus induce the transfer of a piece of bacterial chromosome to a another bacteria |
Is donor living or dead? | YES may be possible, because in this process the active alive donor is not required. | Not possible, because the donor cell have to be active for processing its own DNA. | If the the donor is active during the phage infection then this process may be possible. After infection phage virus kill the becterium and then transfer a piece of Dna to a another bacterium |
Is direct contact required? | Not require. | Must be require direct physical contact with donor to recipient. | Not require. |
Is virus required? | No | no | Yes, only possible after viral infection. |
If DNase present? | Not possible | may be possible because gene transfer involves physical contact so sex pilus is fromed through which transfer process occur. | may be possible. Beacuse virus are aslo enclosed. |
mechanisms of recombination from donor to recipient cell *kind of dna transfered (chromosome, plasmid, or both)...
22. In transformation, transduction or conjugation, what process is necessary for the donor chromosomal DNA to become a part of the recipient cell's chromosome? A. transcription B. conjugation C. gene conversion D. homologous DNA recombination 23. How can the existence of horizontal gene transfer in bacteria affect things like pathogenicity and antibiotic resistance in the world of bacteria? 21. Which type of phages can perform both specialized and generalized transduction? Virulent or Temperate phages? ---- . f ha donar
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
54. The advantage of a cDNA library of eukaryotic genes compared with a genomic library is that the cDNA library: a. always has the entire gene sequence b. lacks introns C. contains both introns and exons d. consists of single-stranded DNA e consists of RNA and DNA 55. Which of the following is an example of a cloning vector? a. Human growth hormone b. Mosquito c. Plasmid d. Tick e. Ribosomal RNA 56. Transformation in recombinant DNA technology: a. Requires...
MUN for the rapid SA can be enormously benehciarlo NS FOR FURTHER REVIEW be found in the answer section at the back wers only after you have attempted to solve th at the back of this d to solve the ques- Answers to these questions can be found in the study guide. Refer to the answers only after you tions ON JONr Own. Multiple Choice 1. DNA genomes are found in: a. All organisms and all viruses b. All organisms,...