part a Estimate the rms speed of an amino acid, whose molecular mass is 89 u, in a living cell at 35 ∘C. part b What would be the rms speed of a protein of molecular mass 85,000 u at 35 ∘C?
part a Estimate the rms speed of an amino acid, whose molecular mass is 89 u,...
Question #4 (10 points) - Mass spectroscopy Amino Acid Molecular Weight 89 174 133 146 137, 194 Alanine Arginine Aspartic acid Glutamic acid Glycine Lysine Proline Serine Tyrosine Valine 105 384 340 Hidal 146 115 105 181 117 146 Mass/charge (here the charge is -1, so it's just mass) Above is shown a mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein...
Assume you were given a mixture consisting of one molecule each of all possible sequences of a smallish protein of molecular weight 4800 daltons. If the average molecular weight of an amino acid is, say, 120 daltons, how much would the sample weigh? How big a container would you need to hold it? What does this calculation tell you about the fraction of possible proteins that are currently in use by living organisms (the average molecular weight of proteins is...
Part C What is the rms speed of an oxygen gas molecule at room temperature (20.0°c)? Atomic mass of oxygen gas molecule - 32.0 u 1 atomic mass uni 1.66 x 10-27 kg m/s
Part C What is the rms speed of an oxygen gas molecule at room temperature (20.0°c)? Atomic mass of oxygen gas molecule - 32.0 u 1 atomic mass uni 1.66 x 10-27 kg m/s
Laboratory studies of protein synthesis often involve the addition of a radioactively labeled amino acid and natural or synthetic RNAs to cell extracts that provide the ribosomes, translation factors, tRNAs etc necessary for translation. Conversion of the labeled amino acid into protein is measured by precipitation using trichloroacetic acid (only proteins precipitate under these conditions). Conversion of amino acids into an acid-precipitable form is measured as a function of time to estimate the rate of protein formation.In the following experiment,...
Use of Modern Molecular Techniques to Determine the Synthetic Pathway of a Novel Amino Acid. Most of the biosynthetic pathways described in our book were determined before the development of recombinant DNA technology and genomics, so the techniques were quite different from those that researchers would use today. Through this question, you will explore an example of the use of modern molecular techniques to investigate the pathway of synthesis of a novel amino acid, (2S)-4- amino-2-hydroxybutyrate (AHBA). AHBA is a...
1. (4 points) Given that the average molecular weight of an amino acid is 110 daltons (Da), determine the following. Remember to convert between daltons and kilodaltons (kDa) where necessary. Each of your answers must be numerically correct and expressed in proper units (1 pt) and show how the value was arrived at using the amino acid molecular weight above (1 pt) for you to receive full credit. A. A molecule of human a-tubulin has a molecular weight of 50.1...
Which of the following would not be facilitated diffusion? A. An amino acid exits the cell through an integral protein. B. A chloride ion (Cl-) unlocks a protein channel, and passes through. C. A large glucose molecule enters the cell through a pore protein. D. A water molecule passes through a semi-permeable membrane.
2.16. Which of the following partial amino acid sequences from a protein whose gene you wish to clone would be most useful in designing an oligonucleotide probe to screen a cDNA library? (a) Met-Leu-Arg-Leu (b) Met-Trp-Cys-Trp Explain why.
1. a) Consider a symporter protein that functions to bring in a specific amino acid into the cell against its gradient. Which of the following ion gradients could this protein use as an energy source? b) Consider an antiporter protein that functions to transport a molecule out of the cell against its gradient. Which of the following ion gradients could this protein use as an energy source? c) Consider a symporter protein that functions to transport a molecule out of...