You need to make a stock of 75% glycerol for archiving phage samples. You locate the undiluted glycerol stock on the shelf. How would you prepare 50ml of the desired glycerol concentration?
We need to make a stock of 75% glycerol, which means in 100 ml 75ml will be glycerol and 25 ml will be water. In order to make 50ml of the desired solution 37.5ml (75*50/100) of glycerol will be added and 12.5ml (50-37.5) of water will be added.
If glycerol existed in a powdered form then for 75% of glycerol -75g will be glycerol powder and 100ml will be water and for 50 ml of the desired solution, 37.5g of glycerol and 50ml of water will be added.
You need to make a stock of 75% glycerol for archiving phage samples. You locate the...
calculate how much glycerol stock you would need to add to make 5ml of a 15% (v/v) glycerol solution (asssume glycerol solution is a liquid offered at 100% w/v)
Once you had obtained a highly concentrated stock of purified phage, how would you separate the recombinant phage DNA away from the phage head and tail proteins?
You start with a 4M stock solution of NaCl, you need to make dilutions to obtain various working solutions. Describe how you would make 100 mL of a working solution that has a final concentration of 1M. Be sure to include the volume and concentration of the components.
How many mLs of each would you need to make 50mL of solution Final concentration of solutes . Stock concentrations MgSO4 0.83 mM MgSO4 1 M CaCl2 0.9 mM CaCl2 1 M KHCO3 0.04 mM KHCO3 0.1 M NaHCO3 0.9 mM NaHCO3 0.5 M Ocean Salts 0.21 g/L Ocean Salts 21 g/L
You are required to make a solution that contains 50 mmoles of buffer (its molecular mass is 121.1 g/mol), 300 mmoles of sodium chloride (its molecular mass is 58.44 g/mol), and 10% glycerol in a total volume of 0.75 L. Tell me (A) what weight of buffer and NaCl you will need, and (B) what volume of glycerol (diluted from a 100% stock concentration) you will need. Also, (C) what then makes up the additional volume to 0.75 L, and...
Hello, I need help answering these two questions. Thank you! 1. How do I make 5 mL of a 1/10 dilution of a solution? 2. How do I figure out the concentration of an undiluted solution(I have the absorbance but the reading is off) if I am unable to use a standard curve?
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
1. You have a stock solution of 35 % hydrogen peroxide. You need to make several dilutions for a reaction. Calculate how much volume of the stock hydrogen peroxide you need to make 50 mL of 10 % hydrogen peroxide. 2. Using the equation of the line from the graph below, calculate the concentration of hydrogen peroxide if 820 mL of foam is produced from the reaction with potassium iodide. Foam Production vs. Concentration H2O2 1500 y 49.534x R-0.9677 1000...
Show how you would make up a 10-5 dilution, given 1 ml pipets and 99ml water blanks. Your original samples is undiluted milk.
. solution. the followi stions, consider a 2.25 M H₂SO4 saut (stock) 1) What is the pH? 2) how would you prepare 150ml of 100M solution - using the stock solution? 3) what is the concentration the concentration of of a K2804 H loome of a 2.00M KOM (as) solution to 75 mL of the stock solution? if noe added. is 4) how much water would you need to add to 100 mc of the stock solution make an aqueous...