Show how you would make up a 10-5 dilution, given 1 ml pipets and 99ml water blanks. Your original samples is undiluted milk.
Show how you would make up a 10-5 dilution, given 1 ml pipets and 99ml water...
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so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
1 You want to make 75 mL of a dilution of disinfectant in water. How much water will you add?n
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
o Section 5 You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given distilled water, test tubes that hold a maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks on the figure to answer this question. mi 5 ml ml ml Iml a ml mi HO Yeast suspension Dilution: Dilution: Dilution: Dilution:
How would you make 1 mL of a 1 to 25 dilution of yeast crude extract? Be specific.
How would you make 120 mL of a 1 to 25 dilution of a yeast extract? I'm really not understanding this process so steps/explanations would be nice. Thanks.
Can you make up 20 ml of a 1:200 dilution from 2 ml of a 1:40 stock solution? (a) If so, how? If not why not? (b) If not what is the largest volume of a 1:200 you could make?